Isolation of transposon insertions.

Transposon insertions in and near a gene of interest facilitate the genetic characterization of a gene in vivo. This chapter is dedicated to describing the isolation of mini-Tn10 insertions in any desired nonessential gene in Salmonella enterica, as well as the isolation of mini-Tn10 insertions near particular genes. The protocols describe use of a tetracycline-resistant Tn10 derivative, but similar approaches can be used for derivatives resistant to other antibiotics. In addition, these approaches are directly applicable to other bacteria that have generalized transducing phages.