Literature DB >> 8655571

Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.

H Li1, J Ruby, N Charon, H Kuramitsu.   

Abstract

Treponema denticola is implicated in the etiology of periodontal diseases. We now report the construction of a specific flgE mutant of T. denticola ATCC 35405 following electroporation utilizing an erythromycin resistance cassette inserted into an flgE DNA fragment. The resulting mutant displays no visible motility and lacks periplasmic flagella as would be predicted from inactivation of the gene for the flagellar hook protein.

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Year:  1996        PMID： 8655571      PMCID： PMC178143          DOI： 10.1128/jb.178.12.3664-3667.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  The collagenolytic activity of Treponema denticola.

Authors:  V J Uitto; D Grenier; Y M Pan; B McBride; T Cawston
Journal:  Matrix Suppl       Date:  1992

Review 2.  Genetics and biogenesis of bacterial flagella.

Authors:  R M Macnab
Journal:  Annu Rev Genet       Date:  1992       Impact factor: 16.830

3.  The role of spirochetes in periodontal disease.

Authors:  W J Loesche
Journal:  Adv Dent Res       Date:  1988-11

4.  Quantitative relationship of Treponema denticola to severity of periodontal disease.

Authors:  L G Simonson; C H Goodman; J J Bial; H E Morton
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

5.  Treponema denticola induces actin rearrangement and detachment of human gingival fibroblasts.

Authors:  P C Baehni; M Song; C A McCulloch; R P Ellen
Journal:  Infect Immun       Date:  1992-08       Impact factor: 3.441

6.  Interaction of Treponema denticola TD-4, GM-1, and MS25 with human gingival fibroblasts.

Authors:  A Weinberg; S C Holt
Journal:  Infect Immun       Date:  1990-06       Impact factor: 3.441

7.  Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.

Authors:  E L Rosey; M J Kennedy; D K Petrella; R G Ulrich; R J Yancey
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

Review 8.  Spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella.

Authors:  N W Charon; E P Greenberg; M B Koopman; R J Limberger
Journal:  Res Microbiol       Date:  1992 Jul-Aug       Impact factor: 3.992

9.  Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis.

Authors:  G R Riviere; K S Elliot; D F Adams; L G Simonson; L B Forgas; A M Nilius; S A Lukehart
Journal:  J Periodontol       Date:  1992-02       Impact factor: 6.993

10.  Pathogen-related oral spirochetes from dental plaque are invasive.

Authors:  G R Riviere; K S Weisz; D F Adams; D D Thomas
Journal:  Infect Immun       Date:  1991-10       Impact factor: 3.441
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  55 in total

Review 1.  Spirochaetal lipoproteins and pathogenesis.

Authors:  D A Haake
Journal:  Microbiology       Date:  2000-07       Impact factor: 2.777

Review 2.  Virulence factors of the oral spirochete Treponema denticola.

Authors:  S G Dashper; C A Seers; K H Tan; E C Reynolds
Journal:  J Dent Res       Date:  2010-10-12       Impact factor: 6.116

3.  Improvement in motion efficiency of the spirochete Brachyspira pilosicoli in viscous environments.

Authors:  S Nakamura; Y Adachi; T Goto; Y Magariyama
Journal:  Biophys J       Date:  2006-01-13       Impact factor: 4.033

4.  Characterization of a methyl-accepting chemotaxis protein gene, dmcA, from the oral spirochete Treponema denticola.

Authors:  M Kataoka; H Li; S Arakawa; H Kuramitsu
Journal:  Infect Immun       Date:  1997-10       Impact factor: 3.441

5.  A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola.

Authors:  Kurni Kurniyati; Weiyan Zhang; Kai Zhang; Chunhao Li
Journal:  Mol Microbiol       Date:  2013-08-01       Impact factor: 3.501

6.  A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola.

Authors:  Kurni Kurniyati; John F Kelly; Evgeny Vinogradov; Anna Robotham; Youbing Tu; Juyu Wang; Jun Liu; Susan M Logan; Chunhao Li
Journal:  Mol Microbiol       Date:  2016-10-27       Impact factor: 3.501

7.  Conservation and revised annotation of the Treponema denticola prcB-prcA-prtP locus encoding the dentilisin (CTLP) protease complex.

Authors:  M P Goetting-Minesky; V Godovikova; J J Li; S Seshadrinathan; J C Timm; S S Kamodia; J C Fenno
Journal:  Mol Oral Microbiol       Date:  2012-12-17       Impact factor: 3.563

8.  Flagellins, but not endoflagellar sheath proteins, of Treponema pallidum and of pathogen-related oral spirochetes are glycosylated.

Authors:  C Wyss
Journal:  Infect Immun       Date:  1998-12       Impact factor: 3.441

9.  Structural analysis of the Leptospiraceae and Borrelia burgdorferi by high-voltage electron microscopy.

Authors:  S F Goldstein; K F Buttle; N W Charon
Journal:  J Bacteriol       Date:  1996-11       Impact factor: 3.490

10.  Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.

Authors:  Linda L Slivienski-Gebhardt; Jacques Izard; William A Samsonoff; Ronald J Limberger
Journal:  Infect Immun       Date:  2004-09       Impact factor: 3.441
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