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Literature DB >> 22247130

Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

Jiang Bian¹, J Christopher Fenno, Chunhao Li.

Abstract

Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.

Entities: Chemical Species

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Year: 2012 PMID： 22247130 PMCID： PMC3298132 DOI： 10.1128/AEM.07461-11

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

30 in total

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Authors: Valentina Godovikova; M Paula Goetting-Minesky; J Christopher Fenno
Journal: Infect Immun Date: 2011-10-10 Impact factor: 3.441

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Journal: Genetics Date: 2000-03 Impact factor: 4.562

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Journal: J Mol Microbiol Biotechnol Date: 2003

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Journal: Infect Immun Date: 1994-08 Impact factor: 3.441

8. Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.

Authors: Linda L Slivienski-Gebhardt; Jacques Izard; William A Samsonoff; Ronald J Limberger
Journal: Infect Immun Date: 2004-09 Impact factor: 3.441

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Journal: J Bacteriol Date: 1984-05 Impact factor: 3.490

10. Cleavage of Treponema denticola PrcA polypeptide to yield protease complex-associated proteins Prca1 and Prca2 is dependent on PrtP.

Authors: Si Young Lee; Xue-Lin Bian; Grace W K Wong; Pauline M Hannam; Barry C McBride; J Christopher Fenno
Journal: J Bacteriol Date: 2002-07 Impact factor: 3.490

14 in total

1. A surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete Treponema denticola.

Authors: Kurni Kurniyati; Weiyan Zhang; Kai Zhang; Chunhao Li
Journal: Mol Microbiol Date: 2013-08-01 Impact factor: 3.501

2. Inactivation of cyclic Di-GMP binding protein TDE0214 affects the motility, biofilm formation, and virulence of Treponema denticola.

Authors: Jiang Bian; Xiangyang Liu; Yi-Qiang Cheng; Chunhao Li
Journal: J Bacteriol Date: 2013-06-21 Impact factor: 3.490

3. pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.

Authors: Kurni Kurniyati; Chunhao Li
Journal: Appl Environ Microbiol Date: 2015-12-18 Impact factor: 4.792

4. Motor rotation is essential for the formation of the periplasmic flagellar ribbon, cellular morphology, and Borrelia burgdorferi persistence within Ixodes scapularis tick and murine hosts.

Authors: Syed Z Sultan; Padmapriya Sekar; Xiaowei Zhao; Akarsh Manne; Jun Liu; R Mark Wooten; M A Motaleb
Journal: Infect Immun Date: 2015-02-17 Impact factor: 3.441

5. Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.

Authors: Yuebin Li; John Ruby; Hui Wu
Journal: Appl Environ Microbiol Date: 2015-04-17 Impact factor: 4.792

6. A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405.

Authors: Valentina Godovikova; M Paula Goetting-Minesky; Jae M Shin; Yvonne L Kapila; Alexander H Rickard; J Christopher Fenno
Journal: Appl Environ Microbiol Date: 2015-07-10 Impact factor: 4.792