Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering.

Literature DB >> 18715002

Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering.

Jessica S Lamb¹, Brian D Zoltowski, Suzette A Pabit, Brian R Crane, Lois Pollack.

Abstract

Time-resolved small-angle X-ray scattering (SAXS) has been used to probe photoexcitation of the blue-light signal transduction protein Vivid (VVD). Laser excitation of sample in a continuous flow cell enables time-resolved measurement of the initial response of VVD to illumination. Good signal-to-noise is achieved without relying on multiple exposures of the same sample or limiting exposure times to prevent radiation damage. The SAXS data demonstrate that VVD dimerizes within tens of milliseconds of light-state activation. Time-resolved SAXS in a flow cell format is a general method for connecting chemical changes in photoreceptors to conformationally driven output signals.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 2008 PMID： 18715002 PMCID： PMC2743002 DOI： 10.1021/ja804236f

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

14 in total

1. Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering.

Authors: L Pollack; M W Tate; N C Darnton; J B Knight; S M Gruner; W A Eaton; R H Austin
Journal: Proc Natl Acad Sci U S A Date: 1999-08-31 Impact factor: 11.205

Review 2. Genetic and molecular analysis of circadian rhythms in Neurospora.

Authors: J J Loros; J C Dunlap
Journal: Annu Rev Physiol Date: 2001 Impact factor: 19.318

3. Conformational changes in a photosensory LOV domain monitored by time-resolved NMR spectroscopy.

Authors: Shannon M Harper; Lori C Neil; Iain J Day; P J Hore; Kevin H Gardner
Journal: J Am Chem Soc Date: 2004-03-24 Impact factor: 15.419

4. The LOV2 domain of phototropin: a reversible photochromic switch.

Authors: John T M Kennis; Ivo H M van Stokkum; Sean Crosson; Magdalena Gauden; Keith Moffat; Rienk van Grondelle
Journal: J Am Chem Soc Date: 2004-04-14 Impact factor: 15.419

5. Structural basis of ARNT PAS-B dimerization: use of a common beta-sheet interface for hetero- and homodimerization.

Authors: Paul B Card; Paul J A Erbel; Kevin H Gardner
Journal: J Mol Biol Date: 2005-09-06 Impact factor: 5.469

6. Stopped-flow x-ray scattering: the dissociation of aspartate transcarbamylase.

Authors: M F Moody; P Vachette; A M Foote; A Tardieu; M H Koch; J Bordas
Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205

7. Light-induced structural changes of LOV domain-containing polypeptides from Arabidopsis phototropin 1 and 2 studied by small-angle X-ray scattering.

Authors: Masayoshi Nakasako; Tatsuya Iwata; Daisuke Matsuoka; Satoru Tokutomi
Journal: Biochemistry Date: 2004-11-30 Impact factor: 3.162

8. VIVID is a flavoprotein and serves as a fungal blue light photoreceptor for photoadaptation.

Authors: Carsten Schwerdtfeger; Hartmut Linden
Journal: EMBO J Date: 2003-09-15 Impact factor: 11.598

9. White Collar-1, a circadian blue light photoreceptor, binding to the frequency promoter.

Authors: Allan C Froehlich; Yi Liu; Jennifer J Loros; Jay C Dunlap
Journal: Science Date: 2002-07-04 Impact factor: 47.728

10. Light activation of the LOV protein vivid generates a rapidly exchanging dimer.

Authors: Brian D Zoltowski; Brian R Crane
Journal: Biochemistry Date: 2008-06-14 Impact factor: 3.162

19 in total

1. High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell.

Authors: S S Nielsen; M Møller; R E Gillilan
Journal: J Appl Crystallogr Date: 2012-02-09 Impact factor: 3.304

2. Spatiotemporal control of gene expression by a light-switchable transgene system.

Authors: Xue Wang; Xianjun Chen; Yi Yang
Journal: Nat Methods Date: 2012-02-12 Impact factor: 28.547

3. Probing fast ribozyme reactions under biological conditions with rapid quench-flow kinetics.

Authors: Jamie L Bingaman; Kyle J Messina; Philip C Bevilacqua
Journal: Methods Date: 2017-03-14 Impact factor: 3.608

4. An analysis of the solution structure and signaling mechanism of LovK, a sensor histidine kinase integrating light and redox signals.

Authors: Erin B Purcell; Claudia A McDonald; Bruce A Palfey; Sean Crosson
Journal: Biochemistry Date: 2010-08-10 Impact factor: 3.162