| Literature DB >> 18692475 |
Mike Boxem1, Zoltan Maliga, Niels Klitgord, Na Li, Irma Lemmens, Miyeko Mana, Lorenzo de Lichtervelde, Joram D Mul, Diederik van de Peut, Maxime Devos, Nicolas Simonis, Muhammed A Yildirim, Murat Cokol, Huey-Ling Kao, Anne-Sophie de Smet, Haidong Wang, Anne-Lore Schlaitz, Tong Hao, Stuart Milstein, Changyu Fan, Mike Tipsword, Kevin Drew, Matilde Galli, Kahn Rhrissorrakrai, David Drechsel, Daphne Koller, Frederick P Roth, Lilia M Iakoucheva, A Keith Dunker, Richard Bonneau, Kristin C Gunsalus, David E Hill, Fabio Piano, Jan Tavernier, Sander van den Heuvel, Anthony A Hyman, Marc Vidal.
Abstract
Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.Entities:
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Year: 2008 PMID： 18692475 PMCID： PMC2596478 DOI： 10.1016/j.cell.2008.07.009
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582