AIM: To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese. METHODS: DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed EC. RESULTS: In control subjects, the frequency of T/T and G/T genotypes, and T and G alleles was 81.4%, 18.1%, 90.05% and 9.55%, respectively. The distribution of genotypes and allelotypes in the EC patients was not significantly different from that in the controls. When stratified by sex and age, there was still no significant association between the risks of EC and GT and GG genotypes. This study also showed a distinct difference in the distribution of DNMT3B and single nucleotide polymorphism (SNP) between Chinese and Koreans. CONCLUSION: DNMT3B 579 G>T polymorphism may not be a stratification marker to predict the susceptibility to EC, at least in Chinese. DNMT3B promoter SNP is diverse in ethnic populations.
AIM: To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese. METHODS:DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed EC. RESULTS: In control subjects, the frequency of T/T and G/T genotypes, and T and G alleles was 81.4%, 18.1%, 90.05% and 9.55%, respectively. The distribution of genotypes and allelotypes in the EC patients was not significantly different from that in the controls. When stratified by sex and age, there was still no significant association between the risks of EC and GT and GG genotypes. This study also showed a distinct difference in the distribution of DNMT3B and single nucleotide polymorphism (SNP) between Chinese and Koreans. CONCLUSION:DNMT3B 579 G>T polymorphism may not be a stratification marker to predict the susceptibility to EC, at least in Chinese. DNMT3B promoter SNP is diverse in ethnic populations.
Authors: Tatiana de Almeida Simão; Gabriela Loureiro De Bonis Almeida Simões; Fabiana Siqueira Ribeiro; Daniela Anhel de Paula Cidade; Nelson Adami Andreollo; Luiz Roberto Lopes; Jacyara Maria Brito Macedo; Rodolfo Acatauassu; Ana Maria Rossini Teixeira; Israel Felzenszwalb; Luis Felipe Ribeiro Pinto; Rodolpho Mattos Albano Journal: Hum Exp Toxicol Date: 2006-09 Impact factor: 2.903
Authors: Arancha Cebrian; Paul D Pharoah; Shahana Ahmed; Santiago Ropero; Mario F Fraga; Paula L Smith; Don Conroy; Robert Luben; Barbara Perkins; Douglas F Easton; Alison M Dunning; Manel Esteller; Bruce A J Ponder Journal: Carcinogenesis Date: 2006-02-25 Impact factor: 4.944
Authors: T Skoog; F M van't Hooft; B Kallin; S Jovinge; S Boquist; J Nilsson; P Eriksson; A Hamsten Journal: Hum Mol Genet Date: 1999-08 Impact factor: 6.150