Kulsom Ahmadi1, Azam Soleimani2, Shiva Irani3, Aliasghar Kiani1, Kourosh Ghanadi4, Zahra Noormohamadi3, Foroozan Sakinejad5. 1. Lorestan University of Medical Sciences, Khorramabad, Iran. 2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. asoleymani12@gmail.com. 3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. 4. Razi Herbal Medicines Research Center and Department of Internal Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 5. Fatemeh Zahra laboratory, Khorramabad, Iran.
Abstract
BACKGROUND: Many studies have suggested that modulation of DNMT3B function caused by single nucleotide polymorphisms of the DNMT3B promoter region may underlie the susceptibility to various cancers such as tumors of the digestive system. The aim of this study was to investigate the effect of -579 G>T polymorphism in the promoter of the DNMT3B gene on risk of gastric cancer in a population from West Iran. PATIENTS AND METHODS: We conducted a case-control study in 100 gastric cancer patients and 112 cancer-free controls to assess the correlation between DNMT3B -579 G>T (rs1569686) polymorphism and the risk of gastric cancer. Detection of genotypes of DNMT3B G39179T polymorphism was analyzed by PCR-RFLP. RESULTS: There was no significant difference in the distribution of DNMT3B -579 G>T genotypes between the cases and controls. However, in the stratified analysis by clinicopathological characteristic types, we found that statistically, the risk susceptibility to gastric cancer was significantly associated with tumor grade II and GT/TT genotype of patients, compared to patients having GG genotype, (OR = 5.4737, 95% CI = 1.4746. 20.3184, P = 0.01). CONCLUSIONS: Our study suggested that the -579 T allele may increase the relative risk for the progression of clinicopathological characteristic of tumor grade of gastric cancer patients.
BACKGROUND: Many studies have suggested that modulation of DNMT3B function caused by single nucleotide polymorphisms of the DNMT3B promoter region may underlie the susceptibility to various cancers such as tumors of the digestive system. The aim of this study was to investigate the effect of -579 G>T polymorphism in the promoter of the DNMT3B gene on risk of gastric cancer in a population from West Iran. PATIENTS AND METHODS: We conducted a case-control study in 100 gastric cancerpatients and 112 cancer-free controls to assess the correlation between DNMT3B -579 G>T (rs1569686) polymorphism and the risk of gastric cancer. Detection of genotypes of DNMT3BG39179T polymorphism was analyzed by PCR-RFLP. RESULTS: There was no significant difference in the distribution of DNMT3B -579 G>T genotypes between the cases and controls. However, in the stratified analysis by clinicopathological characteristic types, we found that statistically, the risk susceptibility to gastric cancer was significantly associated with tumor grade II and GT/TT genotype of patients, compared to patients having GG genotype, (OR = 5.4737, 95% CI = 1.4746. 20.3184, P = 0.01). CONCLUSIONS: Our study suggested that the -579 T allele may increase the relative risk for the progression of clinicopathological characteristic of tumor grade of gastric cancerpatients.
Authors: Gerald N Wogan; Stephen S Hecht; James S Felton; Allan H Conney; Lawrence A Loeb Journal: Semin Cancer Biol Date: 2004-12 Impact factor: 15.707