PURPOSE: DNA methyltransferase-3B (DNMT3B) plays an important role in the generation of aberrant methylation in carcinogenesis. Polymorphisms of the DNMT3B gene may influence DNMT3B enzyme activity on DNA methylation, thereby modulating the susceptibility to colorectal cancer (CRC). METHODS: The polymorphisms in the promoter region of the DNMT3B gene [-149C>T (rs2424913) and -579G>T (rs1569686)] were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and a total of 544 CRC patients and 533 age- and sex-matched healthy controls were enrolled in the case-control study. RESULTS: The results showed that the -579G allele was associated with a significantly decreased risk of CRC (adjusted OR, 0.50; 95%CI, 0.35-0.72; P = 0.0002) when compared with the -579TT genotype. However, the DNMT3B-149CT genotype was not associated with the risk of CRC (adjusted OR, 0.48; 95%CI, 0.18-1.30; P = 0.151). In addition, stratification analysis revealed that the increased risk was predominant in both colon cancer and rectal cancer showing no effect of primary occurrence site. CONCLUSION: Our research demonstrated the -579G allele was a potential protective factor for the occurrence of CRC.
PURPOSE:DNA methyltransferase-3B (DNMT3B) plays an important role in the generation of aberrant methylation in carcinogenesis. Polymorphisms of the DNMT3B gene may influence DNMT3B enzyme activity on DNA methylation, thereby modulating the susceptibility to colorectal cancer (CRC). METHODS: The polymorphisms in the promoter region of the DNMT3B gene [-149C>T (rs2424913) and -579G>T (rs1569686)] were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and a total of 544 CRC patients and 533 age- and sex-matched healthy controls were enrolled in the case-control study. RESULTS: The results showed that the -579G allele was associated with a significantly decreased risk of CRC (adjusted OR, 0.50; 95%CI, 0.35-0.72; P = 0.0002) when compared with the -579TT genotype. However, the DNMT3B-149CT genotype was not associated with the risk of CRC (adjusted OR, 0.48; 95%CI, 0.18-1.30; P = 0.151). In addition, stratification analysis revealed that the increased risk was predominant in both colon cancer and rectal cancer showing no effect of primary occurrence site. CONCLUSION: Our research demonstrated the -579G allele was a potential protective factor for the occurrence of CRC.
Authors: Tatiana de Almeida Simão; Gabriela Loureiro De Bonis Almeida Simões; Fabiana Siqueira Ribeiro; Daniela Anhel de Paula Cidade; Nelson Adami Andreollo; Luiz Roberto Lopes; Jacyara Maria Brito Macedo; Rodolfo Acatauassu; Ana Maria Rossini Teixeira; Israel Felzenszwalb; Luis Felipe Ribeiro Pinto; Rodolpho Mattos Albano Journal: Hum Exp Toxicol Date: 2006-09 Impact factor: 2.903
Authors: Su Jeong Lee; Hyo-Sung Jeon; Jin-Sung Jang; Sun Ha Park; Ga Young Lee; Byung-Heon Lee; Chang Ho Kim; Young Mo Kang; Won Kee Lee; Sin Kam; Rang Woon Park; In-San Kim; Young Lae Cho; Tae Hoon Jung; Jae Yong Park Journal: Carcinogenesis Date: 2004-11-04 Impact factor: 4.944
Authors: J Shawn Jones; Christopher I Amos; Mala Pande; Xiangjun Gu; Jinyun Chen; Imelda M Campos; Qingyi Wei; Miguel Rodriguez-Bigas; Patrick M Lynch; Marsha L Frazier Journal: Cancer Epidemiol Biomarkers Prev Date: 2006-05 Impact factor: 4.254
Authors: Daniel Hernández-Sotelo; Rubén García-Aguilar; Yaneth Castro-Coronel; Jonathan J Magaña; Marco Antonio Leyva-Vazquez; Luz del Carmen Alarcón-Romero; Esther López-Bayghen; Berenice Illades-Aguiar Journal: Mol Biol Rep Date: 2013-05-16 Impact factor: 2.316