Literature DB >> 18341279

Resolution enhancement in solid-state NMR of oriented membrane proteins by anisotropic differential linebroadening.

Thomas Vosegaard1, Kresten Bertelsen, Jan M Pedersen, Lea Thøgersen, Birgit Schiøtt, Emad Tajkhorshid, Troels Skrydstrup, Niels Chr Nielsen.   

Abstract

We demonstrate that a significant improvement in the spectral resolution may be achieved in solid-state NMR experiments of proteins in inhomogeneously disordered oriented lipid bilayers. Using 1H homonuclear decoupling instead of standard 1H heteronuclear decoupling, the 15N line widths may be reduced by up to seven times for such samples. For large oriented membrane proteins, such resolution enhancements may be crucial for assignment and structural interpretation.

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Year:  2008        PMID： 18341279      PMCID： PMC5706113          DOI： 10.1021/ja8000612

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  22 in total

1.  Imaging membrane protein helical wheels.

Authors:  J Wang; J Denny; C Tian; S Kim; Y Mo; F Kovacs; Z Song; K Nishimura; Z Gan; R Fu; J R Quine; T A Cross
Journal:  J Magn Reson       Date:  2000-05       Impact factor: 2.229

Review 2.  Structure determination of membrane proteins by NMR spectroscopy.

Authors:  Stanley J Opella; Francesca M Marassi
Journal:  Chem Rev       Date:  2004-08       Impact factor: 60.622

3.  Optimizing oriented planar-supported lipid samples for solid-state protein NMR.

Authors:  Jan K Rainey; Brian D Sykes
Journal:  Biophys J       Date:  2005-08-05       Impact factor: 4.033

4.  High-resolution conformation of gramicidin A in a lipid bilayer by solid-state NMR.

Authors:  R R Ketchem; W Hu; T A Cross
Journal:  Science       Date:  1993-09-10       Impact factor: 47.728

5.  An improved broadband decoupling sequence for liquid crystals and solids.

Authors:  B M Fung; A K Khitrin; K Ermolaev
Journal:  J Magn Reson       Date:  2000-01       Impact factor: 2.229

6.  Determination of the structure of a membrane-incorporated ion channel. Solid-state nuclear magnetic resonance studies of gramicidin A.

Authors:  R Smith; D E Thomas; F Separovic; A R Atkins; B A Cornell
Journal:  Biophys J       Date:  1989-08       Impact factor: 4.033

7.  Towards high-resolution solid-state NMR on large uniformly 15N- and [13C,15N]-labeled membrane proteins in oriented lipid bilayers.

Authors:  Thomas Vosegaard; Niels Chr Nielsen
Journal:  J Biomol NMR       Date:  2002-03       Impact factor: 2.835

8.  Structural and orientational constraints of bacteriorhodopsin in purple membranes determined by oriented-sample solid-state NMR spectroscopy.

Authors:  Miya Kamihira; Thomas Vosegaard; A James Mason; Suzana K Straus; Niels Chr Nielsen; Anthony Watts
Journal:  J Struct Biol       Date:  2005-01       Impact factor: 2.867

9.  Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5.

Authors:  Ulrich H N Dürr; Kazutoshi Yamamoto; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  J Am Chem Soc       Date:  2007-05-09       Impact factor: 15.419

10.  Tilt and rotational pitch angle of membrane-inserted polypeptides from combined 15N and 2H solid-state NMR spectroscopy.

Authors:  Christopher Aisenbrey; Burkhard Bechinger
Journal:  Biochemistry       Date:  2004-08-17       Impact factor: 3.162
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  5 in total

1.  Peptide aggregation and pore formation in a lipid bilayer: a combined coarse-grained and all atom molecular dynamics study.

Authors:  Lea Thøgersen; Birgit Schiøtt; Thomas Vosegaard; Niels Chr Nielsen; Emad Tajkhorshid
Journal:  Biophys J       Date:  2008-08-01       Impact factor: 4.033

2.  A proton spin diffusion based solid-state NMR approach for structural studies on aligned samples.

Authors:  Jiadi Xu; Pieter E S Smith; Ronald Soong; Ayyalusamy Ramamoorthy
Journal:  J Phys Chem B       Date:  2011-04-05       Impact factor: 2.991

3.  Sensitivity enhancement in static solid-state NMR experiments via single- and multiple-quantum dipolar coherences.

Authors:  T Gopinath; Gianluigi Veglia
Journal:  J Am Chem Soc       Date:  2009-04-29       Impact factor: 15.419

4.  Analysis of the amide (15)N chemical shift tensor of the C(alpha) tetrasubstituted constituent of membrane-active peptaibols, the alpha-aminoisobutyric acid residue, compared to those of di- and tri-substituted proteinogenic amino acid residues.

Authors:  Evgeniy Salnikov; Philippe Bertani; Jan Raap; Burkhard Bechinger
Journal:  J Biomol NMR       Date:  2009-10-11       Impact factor: 2.835

5.  Mechanisms of peptide-induced pore formation in lipid bilayers investigated by oriented 31P solid-state NMR spectroscopy.

Authors:  Kresten Bertelsen; Jerzy Dorosz; Sara Krogh Hansen; Niels Chr Nielsen; Thomas Vosegaard
Journal:  PLoS One       Date:  2012-10-18       Impact factor: 3.240
  5 in total

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