Literature DB >> 18296534

Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide.

Andreas O Wagner1, Cornelia Malin, Brigitte A Knapp, Paul Illmer.   

Abstract

Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.

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Year:  2008        PMID： 18296534      PMCID： PMC2293149          DOI： 10.1128/AEM.02288-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells.

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2.  Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide.

Authors:  Andreas Nocker; Anne K Camper
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

3.  Ability of DNA content and DGGE analysis to reflect the performance condition of an anaerobic biowaste fermenter.

Authors:  Cornelia Malin; Paul Illmer
Journal:  Microbiol Res       Date:  2007-08-31       Impact factor: 5.415

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7.  Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples.

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  36 in total

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2.  Use of propidium monoazide in reverse transcriptase PCR to distinguish between infectious and noninfectious enteric viruses in water samples.

Authors:  Sandhya Parshionikar; Ian Laseke; G Shay Fout
Journal:  Appl Environ Microbiol       Date:  2010-05-14       Impact factor: 4.792

3.  Viability Quantitative PCR Utilizing Propidium Monoazide, Spheroplast Formation, and Campylobacter coli as a Bacterial Model.

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Journal:  Appl Environ Microbiol       Date:  2019-10-01       Impact factor: 4.792

Review 4.  Ecology and exploration of the rare biosphere.

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5.  New perspectives on viable microbial communities in low-biomass cleanroom environments.

Authors:  Parag Vaishampayan; Alexander J Probst; Myron T La Duc; Emilee Bargoma; James N Benardini; Gary L Andersen; Kasthuri Venkateswaran
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6.  Selective quantification of viable Escherichia coli bacteria in biosolids by quantitative PCR with propidium monoazide modification.

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Journal:  Appl Environ Microbiol       Date:  2011-05-20       Impact factor: 4.792

7.  Survival of host-associated bacteroidales cells and their relationship with Enterococcus spp., Campylobacter jejuni, Salmonella enterica serovar Typhimurium, and adenovirus in freshwater microcosms as measured by propidium monoazide-quantitative PCR.

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Journal:  Appl Environ Microbiol       Date:  2011-12-02       Impact factor: 4.792

8.  Optimization, validation, and application of a real-time PCR protocol for quantification of viable bacterial cells in municipal sewage sludge and biosolids using reporter genes and Escherichia coli.

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Journal:  J Ind Microbiol Biotechnol       Date:  2013-11       Impact factor: 3.346

9.  Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.

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10.  Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.

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Journal:  Appl Environ Microbiol       Date:  2009-03-06       Impact factor: 4.792
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