Literature DB >> 19270114

Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.

Sungwoo Bae1, Stefan Wuertz.   

Abstract

Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 microM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

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Year:  2009        PMID： 19270114      PMCID： PMC2681701          DOI： 10.1128/AEM.01333-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  Development of Bacteroides 16S rRNA gene TaqMan-based real-time PCR assays for estimation of total, human, and bovine fecal pollution in water.

Authors:  Alice Layton; Larry McKay; Dan Williams; Victoria Garrett; Randall Gentry; Gary Sayler
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

2.  Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells.

Authors:  Andreas Nocker; Ching-Ying Cheung; Anne K Camper
Journal:  J Microbiol Methods       Date:  2006-06-05       Impact factor: 2.363

3.  Quantification of host-specific Bacteroides-Prevotella 16S rRNA genetic markers for assessment of fecal pollution in freshwater.

Authors:  Satoshi Okabe; Noriko Okayama; Olga Savichtcheva; Tsukasa Ito
Journal:  Appl Microbiol Biotechnol       Date:  2006-12-01       Impact factor: 4.813

4.  Validation of hollow fiber ultrafiltration and real-time PCR using bacteriophage PP7 as surrogate for the quantification of viruses from water samples.

Authors:  Veronica B Rajal; Belinda S McSwain; Donald E Thompson; Christian M Leutenegger; Beverly J Kildare; Stefan Wuertz
Journal:  Water Res       Date:  2007-02-20       Impact factor: 11.236

Review 5.  Quo vadis source tracking? Towards a strategic framework for environmental monitoring of fecal pollution.

Authors:  Jorge W Santo Domingo; Dustin G Bambic; Thomas A Edge; Stefan Wuertz
Journal:  Water Res       Date:  2007-06-06       Impact factor: 11.236

6.  Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide.

Authors:  Andreas O Wagner; Cornelia Malin; Brigitte A Knapp; Paul Illmer
Journal:  Appl Environ Microbiol       Date:  2008-02-22       Impact factor: 4.792

7.  Microbial source tracking: a forensic technique for microbial source identification?

Authors:  Carl M Stapleton; Mark D Wyer; David Kay; John Crowther; Adrian T McDonald; Martin Walters; Andrew Gawler; Terry Hindle
Journal:  J Environ Monit       Date:  2007-04-23

8.  Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples.

Authors:  Knut Rudi; Birgitte Moen; Signe Marie Drømtorp; Askild L Holck
Journal:  Appl Environ Microbiol       Date:  2005-02       Impact factor: 4.792

9.  Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR.

Authors:  Gabriele Flekna; Polonca Stefanic; Martin Wagner; Frans J M Smulders; Sonja Smole Mozina; Ingeborg Hein
Journal:  Res Microbiol       Date:  2007-03-12       Impact factor: 3.992

10.  Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions.

Authors:  Georg H Reischer; David C Kasper; Ralf Steinborn; Robert L Mach; Andreas H Farnleitner
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792
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  45 in total

1.  Unsuitability of quantitative Bacteroidales 16S rRNA gene assays for discerning fecal contamination of drinking water.

Authors:  Paul W J J van der Wielen; Gertjan Medema
Journal:  Appl Environ Microbiol       Date:  2010-05-28       Impact factor: 4.792

2.  Relative decay of Bacteroidales microbial source tracking markers and cultivated Escherichia coli in freshwater microcosms.

Authors:  Linda K Dick; Erin A Stelzer; Erin E Bertke; Denise L Fong; Donald M Stoeckel
Journal:  Appl Environ Microbiol       Date:  2010-03-26       Impact factor: 4.792

3.  Distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using RNA- and DNA-based quantitative PCR assays.

Authors:  Vikram Kapoor; Tarja Pitkänen; Hodon Ryu; Michael Elk; David Wendell; Jorge W Santo Domingo
Journal:  Appl Environ Microbiol       Date:  2014-10-17       Impact factor: 4.792

4.  Emission Sources of Campylobacter from Agricultural Farms, Impact on Environmental Contamination and Intervention Strategies.

Authors:  Vanessa Szott; Anika Friese
Journal:  Curr Top Microbiol Immunol       Date:  2021       Impact factor: 4.291

5.  Metagenomics as a Public Health Risk Assessment Tool in a Study of Natural Creek Sediments Influenced by Agricultural and Livestock Runoff: Potential and Limitations.

Authors:  Brittany Suttner; Eric R Johnston; Luis H Orellana; Luis M Rodriguez-R; Janet K Hatt; Diana Carychao; Michelle Q Carter; Michael B Cooley; Konstantinos T Konstantinidis
Journal:  Appl Environ Microbiol       Date:  2020-03-02       Impact factor: 4.792

6.  Portable platform for rapid in-field identification of human fecal pollution in water.

Authors:  Yu Sherry Jiang; Timothy E Riedel; Jessica A Popoola; Barrett R Morrow; Sheng Cai; Andrew D Ellington; Sanchita Bhadra
Journal:  Water Res       Date:  2017-12-13       Impact factor: 11.236

Review 7.  Microbial invasions in sludge anaerobic digesters.

Authors:  Nuria Fernandez-Gonzalez; G H R Braz; L Regueiro; J M Lema; M Carballa
Journal:  Appl Microbiol Biotechnol       Date:  2020-11-18       Impact factor: 4.813

8.  Continuous Excretion of Leptospira borgpetersenii Ballum in Mice Assessed by Viability Quantitative Polymerase Chain Reaction.

Authors:  Marie-Estelle Soupé-Gilbert; Emilie Bierque; Sophie Geroult; Magali Teurlai; Cyrille Goarant
Journal:  Am J Trop Med Hyg       Date:  2017-07-19       Impact factor: 2.345

9.  Distribution of genetic markers of fecal pollution on a freshwater sandy shoreline in proximity to wastewater effluent.

Authors:  Jessica J Eichmiller; Randall E Hicks; Michael J Sadowsky
Journal:  Environ Sci Technol       Date:  2013-03-21       Impact factor: 9.028

10.  Meeting report: knowledge and gaps in developing microbial criteria for inland recreational waters.

Authors:  Samuel Dorevitch; Nicholas J Ashbolt; Christobel M Ferguson; Roger Fujioka; Charles D McGee; Jeffrey A Soller; Richard L Whitman
Journal:  Environ Health Perspect       Date:  2010-01-25       Impact factor: 9.031
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