| Literature DB >> 18295767 |
Alena Zíková1, Jana Kopecná, Maria A Schumacher, Kenneth Stuart, Lukás Trantírek, Julius Lukes.
Abstract
The mitochondrial RNA-binding proteins (MRP) 1 and 2 play a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Here, we report the purification of a high molecular weight protein complex consisting solely of the MRP1 and MRP2 proteins from the mitochondrion of T. brucei. The MRP1/MRP2 complex natively purified from T. brucei and the one reconstituted in Escherichia coli in vivo bind guide (g) RNAs and pre-mRNAs with dissociation constants in the nanomolar range, and efficiently promote annealing of pre-mRNAs with their cognate gRNAs. In addition, the MRP1/MRP2 complex stimulates annealing between two non-cognate RNA molecules suggesting that along with the cognate duplexes, spuriously mismatched RNA hybrids may be formed at some rate in vivo. A mechanism of catalysed annealing of gRNA/pre-mRNA by the MRP1/MRP2 complex is proposed.Entities:
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Year: 2008 PMID： 18295767 PMCID： PMC2492832 DOI： 10.1016/j.ijpara.2007.12.009
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981