OBJECTIVE: To determine if metabolomic profiling of embryonic development was associated with implantation rates in IVF. DESIGN: Prospective blinded. SETTING: University-affiliated assisted reproductive technology program. PATIENT(S): Unselected assisted reproductive technology population. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Raman-based biospectroscopic metabolomic profiling of spent culture media and delivery rates. RESULTS: Forty-one spent media samples from 19 patients with known reproductive potential (0 or 100% delivery rates of each embryo that implanted) were evaluated. Raman-based metabolomic profiling was used to calculate a viability index for each sample. On day 3, the spent media of embryos with proven reproductive potential (n = 33) demonstrated higher viability indices (0.875 +/- 0.12) than those that failed to implant (0.56 +/- 0.09). Similar findings were present in spent media from embryos transferred on day 5 (n = 8) (-0.40 +/- -0.21 vs. -0.81 +/- -0.08). Receiver operating characteristic curve analyses were used to select thresholds with the greatest ability to discriminate outcomes. Overall diagnostic accuracy for predicting delivery or a failed implantation was 80.5%. CONCLUSIONS: There is a clear relationship between the reproductive potential of human embryos and their modification of their culture media as detected by Raman biospectroscopy-based metabolomic profiling. This technology offers great potential for development as a tool to allow rapid noninvasive assessment of embryonic reproductive potential before transfer.
OBJECTIVE: To determine if metabolomic profiling of embryonic development was associated with implantation rates in IVF. DESIGN: Prospective blinded. SETTING: University-affiliated assisted reproductive technology program. PATIENT(S): Unselected assisted reproductive technology population. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Raman-based biospectroscopic metabolomic profiling of spent culture media and delivery rates. RESULTS: Forty-one spent media samples from 19 patients with known reproductive potential (0 or 100% delivery rates of each embryo that implanted) were evaluated. Raman-based metabolomic profiling was used to calculate a viability index for each sample. On day 3, the spent media of embryos with proven reproductive potential (n = 33) demonstrated higher viability indices (0.875 +/- 0.12) than those that failed to implant (0.56 +/- 0.09). Similar findings were present in spent media from embryos transferred on day 5 (n = 8) (-0.40 +/- -0.21 vs. -0.81 +/- -0.08). Receiver operating characteristic curve analyses were used to select thresholds with the greatest ability to discriminate outcomes. Overall diagnostic accuracy for predicting delivery or a failed implantation was 80.5%. CONCLUSIONS: There is a clear relationship between the reproductive potential of human embryos and their modification of their culture media as detected by Raman biospectroscopy-based metabolomic profiling. This technology offers great potential for development as a tool to allow rapid noninvasive assessment of embryonic reproductive potential before transfer.
Authors: Roberto Romero; Shali Mazaki-Tovi; Edi Vaisbuch; Juan Pedro Kusanovic; Tinnakorn Chaiworapongsa; Ricardo Gomez; Jyh Kae Nien; Bo Hyun Yoon; Moshe Mazor; Jingqin Luo; David Banks; John Ryals; Chris Beecher Journal: J Matern Fetal Neonatal Med Date: 2010-05-26
Authors: Martin D Keltz; Mario Vega; Ido Sirota; Matthew Lederman; Erin L Moshier; Eric Gonzales; Daniel Stein Journal: J Assist Reprod Genet Date: 2013-08-16 Impact factor: 3.412