PURPOSE: To determine if the antioxidant superoxide dismutase-1 (SOD1 or Cu,Zn-SOD) is released by cultured human cleavage-stage embryos and to assess any link between SOD1 and implantation potential. METHODS: Women (n = 91; ≤40 years old) undergoing IVF treatment with transfer of one or two 8-cell embryos that resulted in 0 or 100% implantation were included. Following individual embryo culture, spent medium samples (n = 122) were collected and levels of SOD1 protein were measured by an enzyme-linked immunosorbent assay. SOD1 detection and concentration in embryo spent medium were analyzed in relation to embryo fragmentation and symmetry scores, and implantation (viable fetus at >12 weeks). RESULTS: Cleavage-stage embryos release SOD1 protein into the spent culture medium. Neither detection nor concentration of SOD1 was related to implantation. There was a positive relationship between increased embryo fragmentation scores and SOD1 release, with no apparent association with symmetry. In non-pregnant cycles, the release of SOD1 decreased with increasing maternal age. CONCLUSIONS: While SOD1 does not predict implantation potential of select good-quality embryos, our data support the need to evaluate the biological significance of released SOD1 by embryos of varying quality and from patients of varying age.
PURPOSE: To determine if the antioxidant superoxide dismutase-1 (SOD1 or Cu,Zn-SOD) is released by cultured human cleavage-stage embryos and to assess any link between SOD1 and implantation potential. METHODS:Women (n = 91; ≤40 years old) undergoing IVF treatment with transfer of one or two 8-cell embryos that resulted in 0 or 100% implantation were included. Following individual embryo culture, spent medium samples (n = 122) were collected and levels of SOD1 protein were measured by an enzyme-linked immunosorbent assay. SOD1 detection and concentration in embryo spent medium were analyzed in relation to embryo fragmentation and symmetry scores, and implantation (viable fetus at >12 weeks). RESULTS: Cleavage-stage embryos release SOD1 protein into the spent culture medium. Neither detection nor concentration of SOD1 was related to implantation. There was a positive relationship between increased embryo fragmentation scores and SOD1 release, with no apparent association with symmetry. In non-pregnant cycles, the release of SOD1 decreased with increasing maternal age. CONCLUSIONS: While SOD1 does not predict implantation potential of select good-quality embryos, our data support the need to evaluate the biological significance of released SOD1 by embryos of varying quality and from patients of varying age.
Authors: David E Reichman; Joseph Politch; Elizabeth S Ginsburg; Catherine Racowsky Journal: J Assist Reprod Genet Date: 2010-04-28 Impact factor: 3.412
Authors: C Wrenzycki; P De Sousa; E W Overström; R T Duby; D Herrmann; A J Watson; H Niemann; D O'Callaghan; M P Boland Journal: J Reprod Fertil Date: 2000-01
Authors: A S Lequarré; J M Feugang; O Malhomme; I Donnay; A Massip; F Dessy; A Van Langendonckt Journal: Mol Reprod Dev Date: 2001-01 Impact factor: 2.609