| Literature DB >> 18254385 |
Philip J Lee1, Noah C Helman, Wendell A Lim, Paul J Hung.
Abstract
The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.Entities:
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Year: 2008 PMID： 18254385 DOI： 10.2144/000112673
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993