Literature DB >> 23640166

Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast.

Daphne H E W Huberts1, Sung Sik Lee, Javier Gonzáles, Georges E Janssens, Ima Avalos Vizcarra, Matthias Heinemann.   

Abstract

This protocol describes the production and operation of a microfluidic dissection platform for long-term, high-resolution imaging of budding yeast cells. At the core of this platform is an array of micropads that trap yeast cells in a single focal plane. Newly formed daughter cells are subsequently washed away by a continuous flow of fresh culture medium. In a typical experiment, 50-100 cells can be tracked during their entire replicative lifespan. Apart from aging-related research, the microfluidic platform can also be a valuable tool for other studies requiring the monitoring of single cells over time. Here we provide step-by-step instructions on how to fabricate the silicon wafer mold, how to produce and operate the microfluidic device and how to analyze the obtained data. Production of the microfluidic dissection platform and setting up an aging experiment takes ~7 h.

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Year:  2013        PMID： 23640166     DOI： 10.1038/nprot.2013.060

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  25 in total

1.  A mechanism for asymmetric segregation of age during yeast budding.

Authors:  Zhanna Shcheprova; Sandro Baldi; Stephanie Buvelot Frei; Gaston Gonnet; Yves Barral
Journal:  Nature       Date:  2008-07-27       Impact factor: 49.962

2.  Signal processing by the HOG MAP kinase pathway.

Authors:  Pascal Hersen; Megan N McClean; L Mahadevan; Sharad Ramanathan
Journal:  Proc Natl Acad Sci U S A       Date:  2008-05-14       Impact factor: 11.205

3.  Genes determining yeast replicative life span in a long-lived genetic background.

Authors:  Matt Kaeberlein; Kathryn T Kirkland; Stanley Fields; Brian K Kennedy
Journal:  Mech Ageing Dev       Date:  2005-01-07       Impact factor: 5.432

4.  Dependency of size of Saccharomyces cerevisiae cells on growth rate.

Authors:  C B Tyson; P G Lord; A E Wheals
Journal:  J Bacteriol       Date:  1979-04       Impact factor: 3.490

5.  Molecular phenotyping of aging in single yeast cells using a novel microfluidic device.

Authors:  Zhengwei Xie; Yi Zhang; Ke Zou; Onn Brandman; Chunxiong Luo; Qi Ouyang; Hao Li
Journal:  Aging Cell       Date:  2012-05-17       Impact factor: 9.304

6.  Advances in measuring lifespan in the yeast Saccharomyces cerevisiae.

Authors:  Nadège Minois; Magdalena Frajnt; Chris Wilson; James W Vaupel
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-29       Impact factor: 11.205

Review 7.  Poly(dimethylsiloxane) as a material for fabricating microfluidic devices.

Authors:  J Cooper McDonald; George M Whitesides
Journal:  Acc Chem Res       Date:  2002-07       Impact factor: 22.384

8.  Structural heterogeneity in populations of the budding yeast Saccharomyces cerevisiae.

Authors:  M Vanoni; M Vai; L Popolo; L Alberghina
Journal:  J Bacteriol       Date:  1983-12       Impact factor: 3.490

9.  Mitochondrial network size scaling in budding yeast.

Authors:  Susanne M Rafelski; Matheus P Viana; Yi Zhang; Yee-Hung M Chan; Kurt S Thorn; Phoebe Yam; Jennifer C Fung; Hao Li; Luciano da F Costa; Wallace F Marshall
Journal:  Science       Date:  2012-11-09       Impact factor: 47.728

10.  A microfluidic system for dynamic yeast cell imaging.

Authors:  Philip J Lee; Noah C Helman; Wendell A Lim; Paul J Hung
Journal:  Biotechniques       Date:  2008-01       Impact factor: 1.993
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  14 in total

1.  High-throughput microfluidics to control and measure signaling dynamics in single yeast cells.

Authors:  Anders S Hansen; Nan Hao; Erin K O'Shea
Journal:  Nat Protoc       Date:  2015-07-09       Impact factor: 13.491

Review 2.  Microfluidics: reframing biological enquiry.

Authors:  Todd A Duncombe; Augusto M Tentori; Amy E Herr
Journal:  Nat Rev Mol Cell Biol       Date:  2015-09       Impact factor: 94.444

Review 3.  Review of methods to probe single cell metabolism and bioenergetics.

Authors:  Andreas E Vasdekis; Gregory Stephanopoulos
Journal:  Metab Eng       Date:  2014-10-31       Impact factor: 9.783

4.  Yeast Ataxin-2 Forms an Intracellular Condensate Required for the Inhibition of TORC1 Signaling during Respiratory Growth.

Authors:  Yu-San Yang; Masato Kato; Xi Wu; Athanasios Litsios; Benjamin M Sutter; Yun Wang; Chien-Hsiang Hsu; N Ezgi Wood; Andrew Lemoff; Hamid Mirzaei; Matthias Heinemann; Benjamin P Tu
Journal:  Cell       Date:  2019-04-11       Impact factor: 41.582

5.  Aggregation of the Whi3 protein, not loss of heterochromatin, causes sterility in old yeast cells.

Authors:  Gavin Schlissel; Marek K Krzyzanowski; Fabrice Caudron; Yves Barral; Jasper Rine
Journal:  Science       Date:  2017-03-16       Impact factor: 47.728

6.  Microfluidic Platforms for Yeast-Based Aging Studies.

Authors:  Myeong Chan Jo; Lidong Qin
Journal:  Small       Date:  2016-09-26       Impact factor: 13.281

7.  Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae.

Authors:  Daphne H E W Huberts; Javier González; Sung Sik Lee; Athanasios Litsios; Georg Hubmann; Ernst C Wit; Matthias Heinemann
Journal:  Proc Natl Acad Sci U S A       Date:  2014-07-28       Impact factor: 11.205

8.  Differential scaling between G1 protein production and cell size dynamics promotes commitment to the cell division cycle in budding yeast.

Authors:  Athanasios Litsios; Daphne H E W Huberts; Hanna M Terpstra; Paolo Guerra; Alexander Schmidt; Katarzyna Buczak; Alexandros Papagiannakis; Mattia Rovetta; Johan Hekelaar; Georg Hubmann; Marten Exterkate; Andreas Milias-Argeitis; Matthias Heinemann
Journal:  Nat Cell Biol       Date:  2019-11-04       Impact factor: 28.824

9.  Role of SAGA in the asymmetric segregation of DNA circles during yeast ageing.

Authors:  Annina Denoth-Lippuner; Marek Konrad Krzyzanowski; Catherine Stober; Yves Barral
Journal:  Elife       Date:  2014-11-17       Impact factor: 8.140

10.  The Natural Variation in Lifespans of Single Yeast Cells Is Related to Variation in Cell Size, Ribosomal Protein, and Division Time.

Authors:  Georges E Janssens; Liesbeth M Veenhoff
Journal:  PLoS One       Date:  2016-12-01       Impact factor: 3.240
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