Literature DB >> 18044960

Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses.

Eric A Livesay1, Keqi Tang, Beverley K Taylor, Michael A Buschbach, Derek F Hopkins, Brian L LaMarche, Rui Zhao, Yufeng Shen, Daniel J Orton, Ronald J Moore, Ryan T Kelly, Harold R Udseth, Richard D Smith.   

Abstract

We describe a four-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput liquid chromatography-mass spectrometry (LC-MS(/MS)) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of postcolumn switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 18044960      PMCID： PMC2516349          DOI： 10.1021/ac701727r

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  23 in total

1.  Ternary-column system for high-throughput direct-injection bioanalysis by liquid chromatography/tandem mass spectrometry.

Authors:  Y Q Xia; D B Whigan; M L Powell; M Jemal
Journal:  Rapid Commun Mass Spectrom       Date:  2000       Impact factor: 2.419

2.  High-throughput proteomics using high-efficiency multiple-capillary liquid chromatography with on-line high-performance ESI FTICR mass spectrometry.

Authors:  Y Shen; N Tolić; R Zhao; L Pasa-Tolić; L Li; S J Berger; R Harkewicz; G A Anderson; M E Belov; R D Smith
Journal:  Anal Chem       Date:  2001-07-01       Impact factor: 6.986

3.  Multiple-sprayer tandem mass spectrometry with parallel high flow extraction and parallel separation for high-throughput quantitation in biological fluids.

Authors:  Y Deng; H Zeng; S E Unger; J T Wu
Journal:  Rapid Commun Mass Spectrom       Date:  2001       Impact factor: 2.419

4.  Evaluation of a four-channel multiplexed electrospray triple quadrupole mass spectrometer for the simultaneous validation of LC/MS/MS methods in four different preclinical matrixes.

Authors:  L Yang; T D Mann; D Little; N Wu; R P Clement; P J Rudewicz
Journal:  Anal Chem       Date:  2001-04-15       Impact factor: 6.986

5.  High-efficiency nanoscale liquid chromatography coupled on-line with mass spectrometry using nanoelectrospray ionization for proteomics.

Authors:  Yufeng Shen; Rui Zhao; Scott J Berger; Gordon A Anderson; Nestor Rodriguez; Richard D Smith
Journal:  Anal Chem       Date:  2002-08-15       Impact factor: 6.986

Review 6.  Fast LC/MS in the analysis of small molecules.

Authors:  Philip R Tiller; Leslie A Romanyshyn; Uwe D Neue
Journal:  Anal Bioanal Chem       Date:  2003-08-06       Impact factor: 4.142

Review 7.  Current two-dimensional electrophoresis technology for proteomics.

Authors:  Angelika Görg; Walter Weiss; Michael J Dunn
Journal:  Proteomics       Date:  2004-12       Impact factor: 3.984

8.  Ultrahigh-throughput proteomics using fast RPLC separations with ESI-MS/MS.

Authors:  Yufeng Shen; Richard D Smith; Klaus K Unger; Dipika Kumar; Dieter Lubda
Journal:  Anal Chem       Date:  2005-10-15       Impact factor: 6.986

9.  Chemically etched open tubular and monolithic emitters for nanoelectrospray ionization mass spectrometry.

Authors:  Ryan T Kelly; Jason S Page; Quanzhou Luo; Ronald J Moore; Daniel J Orton; Keqi Tang; Richard D Smith
Journal:  Anal Chem       Date:  2006-11-15       Impact factor: 6.986

10.  Direct analysis of protein complexes using mass spectrometry.

Authors:  A J Link; J Eng; D M Schieltz; E Carmack; G J Mize; D R Morris; B M Garvik; J R Yates
Journal:  Nat Biotechnol       Date:  1999-07       Impact factor: 54.908
View more
  87 in total

1.  Proteomic analysis of bronchoalveolar lavage fluid proteins from mice infected with Francisella tularensis ssp. novicida.

Authors:  Susan M Varnum; Bobbie-Jo M Webb-Robertson; Joel G Pounds; Ronald J Moore; Richard D Smith; Charles W Frevert; Shawn J Skerrett; David Wunschel
Journal:  J Proteome Res       Date:  2012-06-22       Impact factor: 4.466

2.  Enhanced sensitivity for selected reaction monitoring mass spectrometry-based targeted proteomics using a dual stage electrodynamic ion funnel interface.

Authors:  Mahmud Hossain; David T Kaleta; Errol W Robinson; Tao Liu; Rui Zhao; Jason S Page; Ryan T Kelly; Ronald J Moore; Keqi Tang; David G Camp; Wei-Jun Qian; Richard D Smith
Journal:  Mol Cell Proteomics       Date:  2010-04-21       Impact factor: 5.911

3.  Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size.

Authors:  Haizhen Zhang; Kristin E Burnum; Maria L Luna; Brianne O Petritis; Jong-Seo Kim; Wei-Jun Qian; Ronald J Moore; Alejandro Heredia-Langner; Bobbie-Jo M Webb-Robertson; Brian D Thrall; David G Camp; Richard D Smith; Joel G Pounds; Tao Liu
Journal:  Proteomics       Date:  2011-11-04       Impact factor: 3.984

4.  The fungal cultivar of leaf-cutter ants produces specific enzymes in response to different plant substrates.

Authors:  Lily Khadempour; Kristin E Burnum-Johnson; Erin S Baker; Carrie D Nicora; Bobbie-Jo M Webb-Robertson; Richard A White; Matthew E Monroe; Eric L Huang; Richard D Smith; Cameron R Currie
Journal:  Mol Ecol       Date:  2016-10-26       Impact factor: 6.185

5.  An LC-IMS-MS platform providing increased dynamic range for high-throughput proteomic studies.

Authors:  Erin Shammel Baker; Eric A Livesay; Daniel J Orton; Ronald J Moore; William F Danielson; David C Prior; Yehia M Ibrahim; Brian L LaMarche; Anoop M Mayampurath; Athena A Schepmoes; Derek F Hopkins; Keqi Tang; Richard D Smith; Mikhail E Belov
Journal:  J Proteome Res       Date:  2010-02-05       Impact factor: 4.466

6.  Multinozzle emitter array chips for small-volume proteomics.

Authors:  Pan Mao; Rafael Gomez-Sjoberg; Daojing Wang
Journal:  Anal Chem       Date:  2012-12-21       Impact factor: 6.986

7.  Sources of technical variability in quantitative LC-MS proteomics: human brain tissue sample analysis.

Authors:  Paul D Piehowski; Vladislav A Petyuk; Daniel J Orton; Fang Xie; Ronald J Moore; Manuel Ramirez-Restrepo; Anzhelika Engel; Andrew P Lieberman; Roger L Albin; David G Camp; Richard D Smith; Amanda J Myers
Journal:  J Proteome Res       Date:  2013-04-10       Impact factor: 4.466

8.  Proteome of Salmonella Enterica Serotype Typhimurium Grown in a Low Mg/pH Medium.

Authors:  Liang Shi; Charles Ansong; Heather Smallwood; Leah Rommereim; Jason E McDermott; Heather M Brewer; Angela D Norbeck; Ronald C Taylor; Jean K Gustin; Fred Heffron; Richard D Smith; Joshua N Adkins
Journal:  J Proteomics Bioinform       Date:  2009

9.  Mixed-isotope labeling with LC-IMS-MS for characterization of protein-protein interactions by chemical cross-linking.

Authors:  Eric D Merkley; Erin S Baker; Kevin L Crowell; Daniel J Orton; Thomas Taverner; Charles Ansong; Yehia M Ibrahim; Meagan C Burnet; John R Cort; Gordon A Anderson; Richard D Smith; Joshua N Adkins
Journal:  J Am Soc Mass Spectrom       Date:  2013-02-20       Impact factor: 3.109

10.  Cerebrospinal fluid proteome of patients with acute Lyme disease.

Authors:  Thomas E Angel; Jon M Jacobs; Robert P Smith; Mark S Pasternack; Susan Elias; Marina A Gritsenko; Anil Shukla; Edward C Gilmore; Carol McCarthy; David G Camp; Richard D Smith; H Shaw Warren
Journal:  J Proteome Res       Date:  2012-08-31       Impact factor: 4.466
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.