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Literature DB >> 23252432

Multinozzle emitter array chips for small-volume proteomics.

Pan Mao¹, Rafael Gomez-Sjoberg, Daojing Wang.

Abstract

High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Hemoglobins
Silicon

Year: 2012 PMID： 23252432 PMCID： PMC3546512 DOI： 10.1021/ac3032965

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

20 in total

1. High-throughput analysis of hemoglobin from neonates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors: Urban A Kiernan; Jeff A Black; Peter Williams; Randall W Nelson
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Review 2. Mining the plasma proteome for disease applications across seven logs of protein abundance.

Authors: Q Zhang; V Faca; S Hanash
Journal: J Proteome Res Date: 2010-11-29 Impact factor: 4.466

Review 3. On the development of plasma protein biomarkers.

Authors: Silvia Surinova; Ralph Schiess; Ruth Hüttenhain; Ferdinando Cerciello; Bernd Wollscheid; Ruedi Aebersold
Journal: J Proteome Res Date: 2010-12-13 Impact factor: 4.466

4. Capillary-based multi nanoelectrospray emitters: improvements in ion transmission efficiency and implementation with capillary reversed-phase LC-ESI-MS.

Authors: Ryan T Kelly; Jason S Page; Rui Zhao; Wei-Jun Qian; Heather M Mottaz; Keqi Tang; Richard D Smith
Journal: Anal Chem Date: 2007-11-29 Impact factor: 6.986

5. Microfabricated monolithic multinozzle emitters for nanoelectrospray mass spectrometry.

Authors: Woong Kim; Mingquan Guo; Peidong Yang; Daojing Wang
Journal: Anal Chem Date: 2007-04-20 Impact factor: 6.986

6. Mass spectrometry: a tool for enhanced detection of hemoglobin variants.

Authors: Peter Kleinert; Marlis Schmid; Karin Zurbriggen; Oliver Speer; Markus Schmugge; Bernd Roschitzki; Silke S Durka; Urs Leopold; Thomas Kuster; Claus W Heizmann; Hannes Frischknecht; Heinz Troxler
Journal: Clin Chem Date: 2007-10-11 Impact factor: 8.327

7. Cancer biomarkers: closer to delivering on their promise.

Authors: Lance A Liotta; Emanuel Petricoin
Journal: Cancer Cell Date: 2011-09-13 Impact factor: 31.743

8. Lung cancer signatures in plasma based on proteome profiling of mouse tumor models.

Authors: Ayumu Taguchi; Katerina Politi; Sharon J Pitteri; William W Lockwood; Vitor M Faça; Karen Kelly-Spratt; Chee-Hong Wong; Qing Zhang; Alice Chin; Kwon-Sik Park; Gary Goodman; Adi F Gazdar; Julien Sage; Daniela M Dinulescu; Raju Kucherlapati; Ronald A Depinho; Christopher J Kemp; Harold E Varmus; Samir M Hanash
Journal: Cancer Cell Date: 2011-09-13 Impact factor: 31.743

9. Hemoglobin variant analysis via direct surface sampling of dried blood spots coupled with high-resolution mass spectrometry.

Authors: Rebecca L Edwards; Andrew J Creese; Mark Baumert; Paul Griffiths; Josephine Bunch; Helen J Cooper
Journal: Anal Chem Date: 2011-02-22 Impact factor: 6.986

10. Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood.

Authors: Rong Fan; Ophir Vermesh; Alok Srivastava; Brian K H Yen; Lidong Qin; Habib Ahmad; Gabriel A Kwong; Chao-Chao Liu; Juliane Gould; Leroy Hood; James R Heath
Journal: Nat Biotechnol Date: 2008-11-16 Impact factor: 54.908

10 in total

1. Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.

Authors: Yuchao Chen; Pan Mao; Daojing Wang
Journal: Anal Chem Date: 2018-09-07 Impact factor: 6.986

2. Separation-encoded microparticles for single-cell western blotting.

Authors: Burcu Gumuscu; Amy E Herr
Journal: Lab Chip Date: 2019-11-27 Impact factor: 6.799

3. Direct Analysis of Biofluids by Mass Spectrometry with Microfluidic Voltage-Assisted Liquid Desorption Electrospray Ionization.

Authors: Xiangtang Li; Rui Xu; Xin Wei; Hankun Hu; Shulin Zhao; Yi-Ming Liu
Journal: Anal Chem Date: 2017-11-03 Impact factor: 6.986

4. Fast quantification of amino acids by microchip electrophoresis-mass spectrometry.

Authors: Xiangtang Li; Dan Xiao; Talia Sanders; Paul B Tchounwou; Yi-Ming Liu
Journal: Anal Bioanal Chem Date: 2013-08-09 Impact factor: 4.142

5. Biomonitoring of perfluorinated compounds in a drop of blood.

Authors: Pan Mao; Daojing Wang
Journal: Environ Sci Technol Date: 2015-05-22 Impact factor: 9.028

6. A Microstructured Fiber with Defined Borosilicate Regions to Produce a Radial Micronozzle Array for Nanoelectrospray Ionization.

Authors: Y Fu; S Morency; K Bachus; D Simon; T Hutama; G T T Gibson; Y Messaddeq; R D Oleschuk
Journal: Sci Rep Date: 2016-02-19 Impact factor: 4.379