Literature DB >> 17913934

Multicenter trials need to use the same assay for hepatitis C virus viral load determination.

Syria Laperche1, Françoise Bouchardeau, Vincent Thibault, Bruno Pozzetto, Sophie Vallet, Arielle R Rosenberg, Anne-Marie Roque-Afonso, Michèle Gassin, Françoise Stoll-Keller, Pascale Trimoulet, Elyanne Gault, Bruno Chanzy, Bernard Mercier, Michel Branger, Jean-Michel Pawlotsky, Cécile Henquell, Françoise Lunel, Catherine Gaudy-Graffin, Sophie Alain, Marie-Laure Chaix, Gilles Duverlie, Jacques Izopet, Jean-Jacques Lefrère.   

Abstract

This study, involving 20 laboratories and using currently available assays for hepatitis C virus RNA quantification, demonstrated that differences in viral load values are due not to interlaboratory variations but rather to the nature of the assay itself. This underlines the importance of using the same assay in multicenter studies or when monitoring antiviral therapy.

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Year:  2007        PMID： 17913934      PMCID： PMC2168504          DOI： 10.1128/JCM.00825-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Expertise of laboratories in viral load quantification, genotyping, and precore mutant determination for hepatitis B virus in a multicenter study.

Authors:  Syria Laperche; Vincent Thibault; Françoise Bouchardeau; Sophie Alain; Sandrine Castelain; Michelle Gassin; Marie Gueudin; Philippe Halfon; Sylvie Larrat; Françoise Lunel; Michèle Martinot-Peignoux; Bernard Mercier; Jean-Michel Pawlotsky; Bruno Pozzetto; Anne-Marie Roque-Afonso; Françoise Roudot-Thoraval; Karine Sauné; Jean-Jacques Lefrère
Journal:  J Clin Microbiol       Date:  2006-10       Impact factor: 5.948

2.  Real-time PCR assays for hepatitis C virus (HCV) RNA quantitation are adequate for clinical management of patients with chronic HCV infection.

Authors:  Philippe Halfon; Marc Bourlière; Guillaume Pénaranda; Hacène Khiri; Denis Ouzan
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

3.  Multilaboratory comparison of hepatitis C virus viral load assays.

Authors:  A M Caliendo; A Valsamakis; Y Zhou; B Yen-Lieberman; J Andersen; S Young; A Ferreira-Gonzalez; G J Tsongalis; R Pyles; J W Bremer; N S Lurain
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

4.  Verification of an assay for quantification of hepatitis C virus RNA by use of an analyte-specific reagent and two different extraction methods.

Authors:  Michael S Forman; Alexandra Valsamakis
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

5.  Expertise of French laboratories in detection, genotyping, and quantification of hepatitis C virus RNA in serum.

Authors:  Jean-Jacques Lefrère; Françoise Roudot-Thoraval; Françoise Lunel; Sophie Alain; Marie-Laure Chaix; Elisabeth Dussaix; Michèle Gassin; Jacques Izopet; Jean-Michel Pawlotsky; Christopher Payan; Françoise Stoll-Keller; Vincent Thibault; Mary-Anne Trabaud; Dominique Bettinger; Marc Bogard; Michel Branger; Claudine Buffet-Janvresse; Anne Charrois; Christine Defer; Catherine Laffont; Joëlle Lerable; Thierry Levayer; Michèle Martinot-Peignoux; Bernard Mercier; Arielle R Rosenberg
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

6.  Clinical performance of the new rRoche COBAS TaqMan HCV Test and High Pure System for extraction, detection and quantitation of HCV RNA in plasma and serum.

Authors:  Huub C r Gelderblom; Sandra Menting; Marcel G Beld
Journal:  Antivir Ther       Date:  2006

7.  Comparison of conventional PCR with real-time PCR and branched DNA-based assays for hepatitis C virus RNA quantification and clinical significance for genotypes 1 to 5.

Authors:  Christoph Sarrazin; Barbara C Gärtner; Dorothea Sizmann; Rainer Babiel; Ulrike Mihm; Wolf Peter Hofmann; Michael von Wagner; Stefan Zeuzem
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

8.  Improvement of hepatitis C virus RNA polymerase chain reaction through a multicentre quality control study. French Study Group for the Standardization of Hepatitis C Virus PCR.

Authors: 
Journal:  J Virol Methods       Date:  1994-08       Impact factor: 2.014

9.  Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent.

Authors:  James M Barbeau; Jennifer Goforth; Angela M Caliendo; Frederick S Nolte
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

10.  Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies.

Authors:  Syria Laperche; Karine Saune; Paul Dény; Gilles Duverlie; Sophie Alain; Marie-Laure Chaix; Catherine Gaudy; Françoise Lunel; Jean-Michel Pawlotsky; Christopher Payan; Bruno Pozzetto; Catherine Tamalet; Vincent Thibault; Sophie Vallet; Françoise Bouchardeau; Jacques Izopet; Jean-Jacques Lefrère
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948
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  3 in total

1.  Molecular Diagnostics in Transfusion Medicine: In Capillary, on a Chip, in Silico, or in Flight?

Authors:  Henk S P Garritsen; Alex Xiu-Cheng Fan; Daniela Lenz; Horst Hannig; Xiao Yan Zhong; Robert Geffers; Werner Lindenmaier; Kurt E J Dittmar; Bernhard Wörmann
Journal:  Transfus Med Hemother       Date:  2009-05-18       Impact factor: 3.747

2.  Analytical variables influencing the HCV RNA determination by TaqMan real-time PCR in routine clinical laboratory practice.

Authors:  Abida Raza; Zameer Ali; Javaid Irfan; Shahnaz Murtaza; Samina Shakeel
Journal:  Mol Biol Rep       Date:  2012-02-12       Impact factor: 2.316

3.  Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection.

Authors:  Jérôme Caron; Véronique Pène; Laia Tolosa; Maxime Villaret; Eléanor Luce; Angélique Fourrier; Jean-Marie Heslan; Samir Saheb; Eric Bruckert; María José Gómez-Lechón; Tuan Huy Nguyen; Arielle R Rosenberg; Anne Weber; Anne Dubart-Kupperschmitt
Journal:  Stem Cell Res Ther       Date:  2019-07-29       Impact factor: 6.832
  3 in total

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