Literature DB >> 1766440

Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

N Anaya1, M I Roncero.   

Abstract

A transformation system has been developed for Mucor circinelloides, by direct cloning of a wild-type methionine gene that complements the auxotrophic mutation. The marker gene isolated was associated with an autonomous replication sequence (ARS) functional in this zygomycete. Southern hybridisation analyses of transformants showed sequence homology both with vector DNA and with Mucor wild-type DNA. The transformation frequency (up to 6000 per microgram DNA) and the mitotic instability of the transformed cells were studied. The hybridisation pattern of undigested DNA from the transformants suggests that the inserts contain a novel autonomous replication element for this filamentous fungus.

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Year:  1991        PMID： 1766440     DOI： 10.1007/bf00280302

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

Authors:  M J Daboussi; A Djeballi; C Gerlinger; P L Blaiseau; I Bouvier; M Cassan; M H Lebrun; D Parisot; Y Brygoo
Journal:  Curr Genet       Date:  1989-06       Impact factor: 3.886

2.  Transformation of Aspergillus oryzae using the A. niger pyrG gene.

Authors:  I E Mattern; S Unkles; J R Kinghorn; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-12

3.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

4.  Cloning of the riboB locus of Aspergillus nidulans.

Authors:  C E Oakley; C F Weil; P L Kretz; B R Oakley
Journal:  Gene       Date:  1987       Impact factor: 3.688

5.  Behaviour of a replicating mitochondrial DNA sequence from Aspergillus amstelodami in Saccharomyces cerevisiae and Aspergillus nidulans.

Authors:  R K Beri; E L Lewis; G Turner
Journal:  Curr Genet       Date:  1988-06       Impact factor: 3.886

6.  Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans.

Authors:  B R Oakley; J E Rinehart; B L Mitchell; C E Oakley; C Carmona; G L Gray; G S May
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

8.  Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca.

Authors:  J Wöstemeyer; A Burmester; C Weigel
Journal:  Curr Genet       Date:  1987       Impact factor: 3.886

9.  Transformation of Aspergillus niger with the homologous nitrate reductase gene.

Authors:  S E Unkles; E I Campbell; D Carrez; C Grieve; R Contreras; W Fiers; C A Van den Hondel; J R Kinghorn
Journal:  Gene       Date:  1989-05-15       Impact factor: 3.688

10.  Isolation and transformation of the pyruvate kinase gene of Aspergillus nidulans.

Authors:  L de Graaff; H van den Broek; J Visser
Journal:  Curr Genet       Date:  1988-04       Impact factor: 3.886
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  11 in total

1.  Transformation of Mucor circinelloides with autoreplicative vectors containing homologous and heterologous ARS elements and the dominant Cbx(r) carboxine-resistance gene.

Authors:  R Ortiz-Alvarado; G A Gonzalez-Hernandez; J C Torres-Guzman; J F Gutierrez-Corona
Journal:  Curr Microbiol       Date:  2006-02-18       Impact factor: 2.188

2.  Gene replacement and ectopic integration in the zygomycete Mucor circinelloides.

Authors:  J Arnau; P Strøman
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886

3.  Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides.

Authors:  Árpád Csernetics; Eszter Tóth; Anita Farkas; Gábor Nagy; Ottó Bencsik; Csaba Vágvölgyi; Tamás Papp
Journal:  World J Microbiol Biotechnol       Date:  2014-12-11       Impact factor: 3.312

4.  A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes.

Authors:  K F Appel; A M Wolff; J Arnau
Journal:  Mol Genet Genomics       Date:  2004-04-16       Impact factor: 3.291

5.  Circular extrachromosomal DNA codes for a surface protein in the (+) mating type of the zygomycete Absidia glauca.

Authors:  J Hänfler; H Teepe; C Weigel; V Kruft; R Lurz; J Wöstemeyer
Journal:  Curr Genet       Date:  1992-10       Impact factor: 3.886

6.  The SEG1 element: a new DNA region promoting stable mitotic segregation of plasmids in the zygomycete Absidia glauca.

Authors:  A Burmester; A Wöstemeyer; J Arnau; J Wöstemeyer
Journal:  Mol Gen Genet       Date:  1992-11

7.  Development of a system for integrative and stable transformation of the zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer.

Authors:  C B Michielse; K Salim; P Ragas; A F J Ram; B Kudla; B Jarry; P J Punt; C A M J J van den Hondel
Journal:  Mol Genet Genomics       Date:  2004-04-06       Impact factor: 3.291

8.  Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.

Authors:  E P Benito; V Campuzano; M A Lŏpez-Matas; J I De Vicente; A P Eslava
Journal:  Mol Gen Genet       Date:  1995-07-28

9.  Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides.

Authors:  I Nyilasi; K Acs; T Papp; E Nagy; C Vágvölgyi
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.629

10.  Construction of a Recyclable Genetic Marker and Serial Gene Deletions in the Human Pathogenic Mucorales Mucor circinelloides.

Authors:  Alexis Garcia; Gloria Adedoyin; Joseph Heitman; Soo Chan Lee
Journal:  G3 (Bethesda)       Date:  2017-07-05       Impact factor: 3.154
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