Literature DB >> 16475501

Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides.

I Nyilasi1, K Acs, T Papp, E Nagy, C Vágvölgyi.   

Abstract

The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.

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Year:  2005        PMID： 16475501     DOI： 10.1007/BF02931423

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.629


  25 in total

1.  Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for recombinant protein production.

Authors:  Anne Mette Wolff; José Arnau
Journal:  Fungal Genet Biol       Date:  2002-02       Impact factor: 3.495

2.  Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Authors:  N Anaya; M I Roncero
Journal:  Mol Gen Genet       Date:  1991-12

Review 3.  Mucor dimorphism.

Authors:  M Orlowski
Journal:  Microbiol Rev       Date:  1991-06

4.  Gene replacement and ectopic integration in the zygomycete Mucor circinelloides.

Authors:  J Arnau; P Strøman
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886

5.  Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leu1 gene.

Authors:  E A Iturriaga; J M Díaz-Mínguez; E P Benito; M I Alvarez; A P Eslava
Journal:  Curr Genet       Date:  1992-03       Impact factor: 3.886

6.  Dichloran-rose bengal medium for enumeration and isolation of molds from foods.

Authors:  A D King; A D Hocking; J I Pitt
Journal:  Appl Environ Microbiol       Date:  1979-05       Impact factor: 4.792

7.  The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.

Authors:  E E Hood; G L Helmer; R T Fraley; M D Chilton
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

8.  Genetic damage following introduction of DNA in Phycomyces.

Authors:  Irina N Obraztsova; Nicolás Prados; Klaus Holzmann; Javier Avalos; Enrique Cerdá-Olmedo
Journal:  Fungal Genet Biol       Date:  2004-02       Impact factor: 3.495

9.  Malt-yeast extract-sucrose agar, a suitable medium for enumeration and isolation of fungi from silage.

Authors:  I Skaar; H Stenwig
Journal:  Appl Environ Microbiol       Date:  1996-10       Impact factor: 4.792

10.  A DNA transformation-competent Arabidopsis genomic library in Agrobacterium.

Authors:  G R Lazo; P A Stein; R A Ludwig
Journal:  Biotechnology (N Y)       Date:  1991-10
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  6 in total

Review 1.  A silver bullet in a golden age of functional genomics: the impact of Agrobacterium-mediated transformation of fungi.

Authors:  Alexander Idnurm; Andy M Bailey; Timothy C Cairns; Candace E Elliott; Gary D Foster; Giuseppe Ianiri; Junhyun Jeon
Journal:  Fungal Biol Biotechnol       Date:  2017-09-26

2.  Genomic, proteomic, and biochemical analyses of oleaginous Mucor circinelloides: evaluating its capability in utilizing cellulolytic substrates for lipid production.

Authors:  Hui Wei; Wei Wang; John M Yarbrough; John O Baker; Lieve Laurens; Stefanie Van Wychen; Xiaowen Chen; Larry E Taylor; Qi Xu; Michael E Himmel; Min Zhang
Journal:  PLoS One       Date:  2013-09-04       Impact factor: 3.240

3.  Cloning of insertion site flanking sequence and construction of transfer DNA insert mutant library in Stylosanthes colletotrichum.

Authors:  Helong Chen; Caiping Hu; Kexian Yi; Guixiu Huang; Jianming Gao; Shiqing Zhang; Jinlong Zheng; Qiaolian Liu; Jingen Xi
Journal:  PLoS One       Date:  2014-10-31       Impact factor: 3.240

Review 4.  Methods for genetic transformation of filamentous fungi.

Authors:  Dandan Li; Yu Tang; Jun Lin; Weiwen Cai
Journal:  Microb Cell Fact       Date:  2017-10-03       Impact factor: 5.328

Review 5.  Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.

Authors:  Hassan Mohamed; Tahira Naz; Junhuan Yang; Aabid Manzoor Shah; Yusuf Nazir; Yuanda Song
Journal:  J Fungi (Basel)       Date:  2021-12-10

6.  Tricarboxylate Citrate Transporter of an Oleaginous Fungus Mucor circinelloides WJ11: From Function to Structure and Role in Lipid Production.

Authors:  Wu Yang; Aabid Manzoor Shah; Shiqi Dong; Caili Sun; Huaiyuan Zhang; Hassan Mohamed; Xiuzhen Gao; Huirong Fan; Yuanda Song
Journal:  Front Nutr       Date:  2021-12-09
  6 in total

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