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Literature DB >> 3502943

Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca.

Abstract

A plasmid (pAmN61) containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts. Neomycin resistant transformants could be selected for on complete medium containing 1.2 mg/ml neomycin sulfate. The physical presence of plasmid DNA in Absidia glauca was demonstrated by retransformation into Escherichia coli and by Southern blot analysis. No integration of plasmid DNA at either one of the two actin loci was observed; Southern blot experiments provide evidence that pAmN61 is autonomously replicated in Absidia glauca.

Entities: Chemical Disease Species

Mesh：

Substances：
Neomycin

Year: 1987 PMID： 3502943 DOI： 10.1007/BF00368066

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

13 in total

1. In vitro packaging of lambda and cosmid DNA.

Authors: B Hohn
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca.

Authors: J Wöstemeyer
Journal: Eur J Biochem Date: 1985-01-15

4. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors: W D Benton; R W Davis
Journal: Science Date: 1977-04-08 Impact factor: 47.728

5. A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.

Authors: P H Schreier; R Cortese
Journal: J Mol Biol Date: 1979-03-25 Impact factor: 5.469

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

7. Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.

Authors: J L Revuelta; M Jayaram
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

9. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

10. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

Authors: G Tschumper; J Carbon
Journal: Gene Date: 1980-07 Impact factor: 3.688

18 in total

1. Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.

Authors: J Arnau; L P Jepsen; P Strøman
Journal: Mol Gen Genet Date: 1991-02

2. Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Authors: N Anaya; M I Roncero
Journal: Mol Gen Genet Date: 1991-12

Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca.

1. In vitro packaging of lambda and cosmid DNA.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca.

4. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

5. A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.

6. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

7. Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

9. Transformation of yeast.

10. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

1. Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.

2. Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

3. Inhibition of non-homologous end joining and integration of DNA upon transformation of Rhizopus oryzae.

4. Gene replacement and ectopic integration in the zygomycete Mucor circinelloides.

5. A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes.

6. Transfer of genetic information from the mycoparasite Parasitella parasitica to its host Absidia glauca.

7. Repair of plasmid DNA used for transformation of Rhizopus oryzae by gene conversion.

8. Characterisation of the Mucor circinelloides regulated promoter gpd1P.

9. A nucleotide sequence involved in replicative transformation of a filamentous fungus.

10. Circular extrachromosomal DNA codes for a surface protein in the (+) mating type of the zygomycete Absidia glauca.