Literature DB >> 3481025

Transformation of Aspergillus oryzae using the A. niger pyrG gene.

I E Mattern1, S Unkles, J R Kinghorn, P H Pouwels, C A van den Hondel.   

Abstract

A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.

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Year:  1987        PMID： 3481025     DOI： 10.1007/BF00327197

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  9 in total

1.  Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors:  P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1987       Impact factor: 3.688

2.  Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae.

Authors:  A Gatignol; M Baron; G Tiraby
Journal:  Mol Gen Genet       Date:  1987-05

3.  A system for the analysis of expression signals in Aspergillus.

Authors:  R F van Gorcom; P J Punt; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1986       Impact factor: 3.688

4.  Development of a high-frequency transforming vector for Aspergillus nidulans.

Authors:  D J Ballance; G Turner
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  Expression of an Escherichia coli beta-galactosidase fusion gene in Aspergillus nidulans.

Authors:  R F van Gorcom; P H Pouwels; T Goosen; J Visser; H W van den Broek; J E Hamer; W E Timberlake; C A van den Hondel
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

7.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

8.  Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans.

Authors:  F P Buxton; D I Gwynne; R W Davies
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors:  J M Kelly; M J Hynes
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
  9 in total
  15 in total

1.  Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Authors:  N Anaya; M I Roncero
Journal:  Mol Gen Genet       Date:  1991-12

2.  Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.

Authors:  I N Roberts; R P Oliver; P J Punt; C A van den Hondel
Journal:  Curr Genet       Date:  1989-03       Impact factor: 3.886

3.  A new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus Aspergillus oryzae using Agrobacterium tumefaciens-mediated transformation.

Authors:  Khuyen Thi Nguyen; Quynh Ngoc Ho; Loc Thi Binh Xuan Do; Linh Thi Dam Mai; Duc-Ngoc Pham; Huyen Thi Thanh Tran; Diep Hong Le; Huy Quang Nguyen; Van-Tuan Tran
Journal:  World J Microbiol Biotechnol       Date:  2017-05-02       Impact factor: 3.312

4.  Transformation of Aspergillus flavus to study aflatoxin biosynthesis.

Authors:  G A Payne; C P Woloshuk
Journal:  Mycopathologia       Date:  1989-09       Impact factor: 2.574

5.  Investigating dominant selection markers for Coprinopsis cinerea: a carboxin resistance system and re-evaluation of hygromycin and phleomycin resistance vectors.

Authors:  Sreedhar Kilaru; Catherine M Collins; Amanda J Hartley; Claire Burns; Gary D Foster; Andy M Bailey
Journal:  Curr Genet       Date:  2009-07-28       Impact factor: 3.886

6.  A trial of minimization of chromosome 7 in Aspergillus oryzae by multiple chromosomal deletions.

Authors:  Feng Jie Jin; Tadashi Takahashi; Michiyo Utsushikawa; Toshi Furukido; Michiyo Nishida; Masahiro Ogawa; Masahumi Tokuoka; Yasuji Koyama
Journal:  Mol Genet Genomics       Date:  2009-10-24       Impact factor: 3.291

7.  Transformation frequencies are enhanced and vector DNA is targeted during retransformation of Leptosphaeria maculans, a fungal plant pathogen.

Authors:  M L Farman; R P Oliver
Journal:  Mol Gen Genet       Date:  1992-01

8.  Cloning, characterization and overproduction of nuclease S1 gene (nucS) from Aspergillus oryzae.

Authors:  B R Lee; K Kitamoto; O Yamada; C Kumagai
Journal:  Appl Microbiol Biotechnol       Date:  1995-12       Impact factor: 4.813

9.  Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.

Authors:  E P Benito; V Campuzano; M A Lŏpez-Matas; J I De Vicente; A P Eslava
Journal:  Mol Gen Genet       Date:  1995-07-28

10.  A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae.

Authors:  Y M de Ruiter-Jacobs; M Broekhuijsen; S E Unkles; E I Campbell; J R Kinghorn; R Contreras; P H Pouwels; C A van den Hondel
Journal:  Curr Genet       Date:  1989-09       Impact factor: 3.886
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