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Literature DB >> 17494764

Single-molecule mass spectrometry in solution using a solitary nanopore.

Joseph W F Robertson¹, Claudio G Rodrigues, Vincent M Stanford, Kenneth A Rubinson, Oleg V Krasilnikov, John J Kasianowicz.

Abstract

We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single alpha-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.

Entities: Chemical

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Year: 2007 PMID： 17494764 PMCID： PMC1866312 DOI： 10.1073/pnas.0611085104

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

Single-molecule mass spectrometry in solution using a solitary nanopore.

1. Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.

2. Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter.

3. Ion-beam sculpting at nanometre length scales.

4. Driven DNA transport into an asymmetric nanometer-scale pore.

5. A simple method for the determination of the pore radius of ion channels in planar lipid bilayer membranes.

6. Translocation of double-strand DNA through a silicon oxide nanopore.

7. Orientation discrimination of single-stranded DNA inside the alpha-hemolysin membrane channel.

8. Transport of maltodextrins through maltoporin: a single-channel study.

9. Characterization of individual polynucleotide molecules using a membrane channel.

10. Partitioning of a polymer into a nanoscopic protein pore obeys a simple scaling law.

1. Stochastic sensing of proteins with receptor-modified solid-state nanopores.

Review 2. Applications of biological pores in nanomedicine, sensing, and nanoelectronics.

3. Using a nanopore for single molecule detection and single cell transfection.

4. Deciphering ionic current signatures of DNA transport through a nanopore.

5. Passage times for polymer translocation pulled through a narrow pore.

6. Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge.

7. Electrical characterization of protein molecules by a solid-state nanopore.

8. Polymers pushing Polymers: Polymer Mixtures in Thermodynamic Equilibrium with a Pore.

9. Molecular dynamics studies of polyethylene oxide and polyethylene glycol: hydrodynamic radius and shape anisotropy.

10. The Role of Lipid Interactions in Simulations of the α-Hemolysin Ion-Channel-Forming Toxin.