OBJECTIVES: The aim of the present pilot study was to investigate initial and early tissue reactions to modified (mod) and conventional sand-blasted, large grit and acid-etched (SLA) titanium implants. MATERIAL AND METHODS: Implantation of modSLA and SLA implants was performed bilaterally in both the mandible and maxilla of dogs. The animals were sacrificed after a healing period of 1, 4, 7 and 14 days, respectively. Peri-implant tissue reactions were assessed in non-decalcified tissue sections using conventional histology (Toluidine blue-TB and Masson Goldner Trichrome stain-MG) and immunohistochemistry using monoclonal antibodies to transglutaminase II (TG) (angiogenesis) and osteocalcin (OC). Bone density (BD) and bone to implant contact (BIC) were assessed histomorphometrically. RESULTS: Day 1 revealed an early TG antigen reactivity in the provisional fibrin matrix adjacent to both implant surfaces. Day 4 was characterized by the formation of a collagen-rich connective tissue (MG), which revealed the first signs of OC synthesis adjacent to modSLA surfaces. Immunohistochemical staining for TG revealed a direct correlation between angiogenesis and new bone formation, which was clearly identifiable after 7 days by means of increasing BD, BIC and OC values. After 14 days, modSLA surfaces seemed to be surrounded by a firmly attached mature, parallel-fibered woven bone. CONCLUSIONS: Within the limits of the present study, it might be concluded that the combination of immunohistochemical and conventional histological stainings in non-decalcified tissue sections is a valuable technique to evaluate the initial and early stages of wound healing around endosseous titanium implants.
OBJECTIVES: The aim of the present pilot study was to investigate initial and early tissue reactions to modified (mod) and conventional sand-blasted, large grit and acid-etched (SLA) titanium implants. MATERIAL AND METHODS: Implantation of modSLA and SLA implants was performed bilaterally in both the mandible and maxilla of dogs. The animals were sacrificed after a healing period of 1, 4, 7 and 14 days, respectively. Peri-implant tissue reactions were assessed in non-decalcified tissue sections using conventional histology (Toluidine blue-TB and Masson Goldner Trichrome stain-MG) and immunohistochemistry using monoclonal antibodies to transglutaminase II (TG) (angiogenesis) and osteocalcin (OC). Bone density (BD) and bone to implant contact (BIC) were assessed histomorphometrically. RESULTS: Day 1 revealed an early TG antigen reactivity in the provisional fibrin matrix adjacent to both implant surfaces. Day 4 was characterized by the formation of a collagen-rich connective tissue (MG), which revealed the first signs of OC synthesis adjacent to modSLA surfaces. Immunohistochemical staining for TG revealed a direct correlation between angiogenesis and new bone formation, which was clearly identifiable after 7 days by means of increasing BD, BIC and OC values. After 14 days, modSLA surfaces seemed to be surrounded by a firmly attached mature, parallel-fibered woven bone. CONCLUSIONS: Within the limits of the present study, it might be concluded that the combination of immunohistochemical and conventional histological stainings in non-decalcified tissue sections is a valuable technique to evaluate the initial and early stages of wound healing around endosseous titanium implants.
Authors: Frank Schwarz; Monika Herten; Martin Sager; Marco Wieland; Michel Dard; Jürgen Becker Journal: Clin Oral Investig Date: 2007-03-15 Impact factor: 3.573
Authors: Frank Schwarz; Ilja Mihatovic; Vladimir Golubovic; Jürgen Becker; Martin Sager Journal: Clin Oral Investig Date: 2013-11-24 Impact factor: 3.573