Literature DB >> 17393201

Use of pyrosequencing to identify point mutations in domain V of 23S rRNA genes of linezolid-resistant Staphylococcus aureus and Staphylococcus epidermidis.

W Zhu1, F C Tenover, J Limor, D Lonsway, D Prince, W M Dunne, J B Patel.   

Abstract

A pyrosequencing assay was used for the rapid characterization of linezolid-resistant isolates of Staphylococcus aureus and Staphylococcus epidermidis. The assay identified base substitutions in copies of the 23S rRNA gene and determined the percentage of alleles with the mutation. Modifications of the assay were necessary to identify all mutations in the 23S rRNA genes of S. epidermidis that were associated with linezolid resistance. A C2534T mutation was identified in S. epidermidis that was not previously reported in a linezolid-resistant isolate.

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Year:  2007        PMID: 17393201     DOI: 10.1007/s10096-007-0261-0

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN: 0934-9723            Impact factor:   3.267


  13 in total

1.  Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.

Authors:  Alistair Sinclair; Catherine Arnold; Neil Woodford
Journal:  Antimicrob Agents Chemother       Date:  2003-11       Impact factor: 5.191

2.  Linezolid resistance in clinical isolates of Staphylococcus aureus.

Authors:  Peter Wilson; J A Andrews; R Charlesworth; R Walesby; M Singer; D J Farrell; M Robbins
Journal:  J Antimicrob Chemother       Date:  2003-01       Impact factor: 5.790

3.  Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis.

Authors:  S H Marshall; C J Donskey; R Hutton-Thomas; R A Salata; L B Rice
Journal:  Antimicrob Agents Chemother       Date:  2002-10       Impact factor: 5.191

4.  Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs.

Authors:  Kathleen A Ruggero; Laura K Schroeder; Paul C Schreckenberger; Alexander S Mankin; John P Quinn
Journal:  Diagn Microbiol Infect Dis       Date:  2003-11       Impact factor: 2.803

5.  Emerging linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolated from two Austrian patients in the same intensive care unit.

Authors:  A P Johnson; L Tysall; M V Stockdale; N Woodford; M E Kaufmann; M Warner; D M Livermore; F Asboth; F J Allerberger
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2002-09-13       Impact factor: 3.267

6.  Resistance to linezolid: characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci.

Authors:  J Prystowsky; F Siddiqui; J Chosay; D L Shinabarger; J Millichap; L R Peterson; G A Noskin
Journal:  Antimicrob Agents Chemother       Date:  2001-07       Impact factor: 5.191

7.  Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain.

Authors:  Steven R Gill; Derrick E Fouts; Gordon L Archer; Emmanuel F Mongodin; Robert T Deboy; Jacques Ravel; Ian T Paulsen; James F Kolonay; Lauren Brinkac; Mauren Beanan; Robert J Dodson; Sean C Daugherty; Ramana Madupu; Samuel V Angiuoli; A Scott Durkin; Daniel H Haft; Jessica Vamathevan; Hoda Khouri; Terry Utterback; Chris Lee; George Dimitrov; Lingxia Jiang; Haiying Qin; Jan Weidman; Kevin Tran; Kathy Kang; Ioana R Hance; Karen E Nelson; Claire M Fraser
Journal:  J Bacteriol       Date:  2005-04       Impact factor: 3.490

8.  Distribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates.

Authors:  Corinna Kehrenberg; Stefan Schwarz
Journal:  Antimicrob Agents Chemother       Date:  2006-04       Impact factor: 5.191

9.  Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations.

Authors:  P Sander; L Belova; Y G Kidan; P Pfister; A S Mankin; E C Böttger
Journal:  Mol Microbiol       Date:  2002-12       Impact factor: 3.501

10.  Linezolid resistance in Staphylococcus aureus: characterization and stability of resistant phenotype.

Authors:  Satish K Pillai; George Sakoulas; Christine Wennersten; George M Eliopoulos; Robert C Moellering; Mary Jane Ferraro; Howard S Gold
Journal:  J Infect Dis       Date:  2002-11-04       Impact factor: 5.226

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  13 in total

1.  Sequential linezolid-resistant Staphylococcus epidermidis isolates with G2576T mutation.

Authors:  Tao Hong; Xiangyang Li; Jie Wang; Cynthia Sloan; Cristina Cicogna
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

2.  Accuracy of six antimicrobial susceptibility methods for testing linezolid against staphylococci and enterococci.

Authors:  Fred C Tenover; Portia P Williams; Sheila Stocker; Angela Thompson; Leigh Ann Clark; Brandi Limbago; Roberta B Carey; Susan M Poppe; Dean Shinabarger; John E McGowan
Journal:  J Clin Microbiol       Date:  2007-07-18       Impact factor: 5.948

3.  Linezolid resistance in Staphylococcus aureus: gene dosage effect, stability, fitness costs, and cross-resistances.

Authors:  Silke Besier; Albrecht Ludwig; Johannes Zander; Volker Brade; Thomas A Wichelhaus
Journal:  Antimicrob Agents Chemother       Date:  2008-01-22       Impact factor: 5.191

4.  The genetic environment of the cfr gene and the presence of other mechanisms account for the very high linezolid resistance of Staphylococcus epidermidis isolate 426-3147L.

Authors:  Jacqueline LaMarre; Rodrigo E Mendes; Teresa Szal; Stefan Schwarz; Ronald N Jones; Alexander S Mankin
Journal:  Antimicrob Agents Chemother       Date:  2012-12-17       Impact factor: 5.191

5.  Endemic linezolid-resistant Staphylococcus epidermidis in a critical care unit.

Authors:  M Treviño; L Martínez-Lamas; P A Romero-Jung; J M Giráldez; J Alvarez-Escudero; B J Regueiro
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-11-05       Impact factor: 3.267

6.  Polyphyletic emergence of linezolid-resistant staphylococci in the United States.

Authors:  Agnes Wong; Shilpa P Reddy; Davida S Smyth; Maria E Aguero-Rosenfeld; George Sakoulas; D Ashley Robinson
Journal:  Antimicrob Agents Chemother       Date:  2009-11-23       Impact factor: 5.191

7.  Linezolid-dependent function and structure adaptation of ribosomes in a Staphylococcus epidermidis strain exhibiting linezolid dependence.

Authors:  Sofia Kokkori; Maria Apostolidi; Athanassios Tsakris; Spyros Pournaras; Constantinos Stathopoulos; George Dinos
Journal:  Antimicrob Agents Chemother       Date:  2014-06-02       Impact factor: 5.191

8.  Emergence of linezolid resistance in a clinical Staphylococcus capitis isolate from Jiangsu Province of China in 2012.

Authors:  Yiling Huang; Yanling Xu; Genyan Liu; Yaning Mei; Wenying Xia; Ting Xu; Bing Gu; Shiyang Pan
Journal:  J Thorac Dis       Date:  2014-05       Impact factor: 2.895

9.  Genomic and Phenotypic Analysis of Linezolid-Resistant Staphylococcus epidermidis in a Tertiary Hospital in Innsbruck, Austria.

Authors:  Silke Huber; Miriam A Knoll; Michael Berktold; Reinhard Würzner; Anita Brindlmayer; Viktoria Weber; Andreas E Posch; Katharina Mrazek; Sarah Lepuschitz; Michael Ante; Stephan Beisken; Dorothea Orth-Höller; Johannes Weinberger
Journal:  Microorganisms       Date:  2021-05-10

10.  Linezolid dependence in Staphylococcus epidermidis bloodstream isolates.

Authors:  Spyros Pournaras; Eleni Ntokou; Olympia Zarkotou; Kyriaki Ranellou; Katerina Themeli-Digalaki; Constantinos Stathopoulos; Athanassios Tsakris
Journal:  Emerg Infect Dis       Date:  2013-01       Impact factor: 6.883

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