Literature DB >> 17142910

Combining site-specific mutagenesis and seeding as a strategy to crystallize 'difficult' proteins: the case of Staphylococcus aureus thioredoxin.

Goedele Roos1, Elke Brosens, Khadija Wahni, Aline Desmyter, Silvia Spinelli, Lode Wyns, Joris Messens, Remy Loris.   

Abstract

The P31T mutant of Staphylococcus aureus thioredoxin crystallizes spontaneously in space group P2(1)2(1)2(1), with unit-cell parameters a = 41.7, b = 49.5, c = 55.6 A. The crystals diffract to 2.2 A resolution. Isomorphous crystals of wild-type thioredoxin as well as of other point mutants only grow when seeded with the P31T mutant. These results suggest seeding as a valuable tool complementing surface engineering for proteins that are hard to crystallize.

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Year:  2006        PMID： 17142910      PMCID： PMC2225371          DOI： 10.1107/S1744309106047075

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  24 in total

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Authors:  A D'Arcy; M Stihle; D Kostrewa; G Dale
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-09

2.  The impact of Lys-->Arg surface mutations on the crystallization of the globular domain of RhoGDI.

Authors:  Jan Czepas; Yancho Devedjiev; Daniel Krowarsch; Urszula Derewenda; Jacek Otlewski; Zygmunt S Derewenda
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-01-23

3.  Rational protein crystallization by mutational surface engineering.

Authors:  Zygmunt S Derewenda
Journal:  Structure       Date:  2004-04       Impact factor: 5.006

4.  Solving the structure of human H ferritin by genetically engineering intermolecular crystal contacts.

Authors:  D M Lawson; P J Artymiuk; S J Yewdall; J M Smith; J C Livingstone; A Treffry; A Luzzago; S Levi; P Arosio; G Cesareni
Journal:  Nature       Date:  1991-02-07       Impact factor: 49.962

5.  Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution.

Authors:  A Holmgren; B O Söderberg; H Eklund; C I Brändén
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

6.  Crystal structure of the obese protein leptin-E100.

Authors:  F Zhang; M B Basinski; J M Beals; S L Briggs; L M Churgay; D K Clawson; R D DiMarchi; T C Furman; J E Hale; H M Hsiung; B E Schoner; D P Smith; X Y Zhang; J P Wery; R W Schevitz
Journal:  Nature       Date:  1997-05-08       Impact factor: 49.962

7.  The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.

Authors:  Agnieszka Mateja; Yancho Devedjiev; Daniel Krowarsch; Kenton Longenecker; Zbigniew Dauter; Jacek Otlewski; Zygmunt S Derewenda
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-11-23

8.  Kinetics and active site dynamics of Staphylococcus aureus arsenate reductase.

Authors:  Joris Messens; José C Martins; Elke Brosens; Karolien Van Belle; Doris M Jacobs; Rudolph Willem; Lode Wyns
Journal:  J Biol Inorg Chem       Date:  2001-07-24       Impact factor: 3.358

9.  How thioredoxin can reduce a buried disulphide bond.

Authors:  Joris Messens; Inge Van Molle; Peter Vanhaesebrouck; Maya Limbourg; Karolien Van Belle; Khadija Wahni; José C Martins; Remy Loris; Lode Wyns
Journal:  J Mol Biol       Date:  2004-06-04       Impact factor: 5.469

10.  Occurrence of glutathione in bacteria.

Authors:  R C Fahey; W C Brown; W B Adams; M B Worsham
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490
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  1 in total

1.  Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7.

Authors:  Pieter De Bruyn; Maruša Prolič-Kalinšek; Alexandra Vandervelde; Milan Malfait; Yann G J Sterckx; Frank Sobott; San Hadži; Els Pardon; Jan Steyaert; Remy Loris
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2021-09-21       Impact factor: 1.072
  1 in total

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