Literature DB >> 17142901

Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase.

Dashuang Shi1, Ljubica Caldovic, Zhongmin Jin, Xiaolin Yu, Qiuhao Qu, Lauren Roth, Hiroki Morizono, Yetrib Hathout, Norma M Allewell, Mendel Tuchman.   

Abstract

A novel N-acetylglutamate synthase/kinase bifunctional enzyme of arginine biosynthesis that was homologous to vertebrate N-acetylglutamate synthases was identified in Xanthomonas campestris. The protein was overexpressed, purified and crystallized. The crystals belong to the hexagonal space group P6(2)22, with unit-cell parameters a = b = 134.60, c = 192.11 A, and diffract to about 3.0 A resolution. Selenomethionine-substituted recombinant protein was produced and selenomethionine substitution was verified by mass spectroscopy. Multiple anomalous dispersion (MAD) data were collected at three wavelengths at SER-CAT, Advanced Photon Source, Argonne National Laboratory. Structure determination is under way using the MAD phasing method.

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Year:  2006        PMID： 17142901      PMCID： PMC2225375          DOI： 10.1107/S1744309106044101

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  26 in total

1.  Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis.

Authors:  Santiago Ramón-Maiques; Alberto Marina; Fernando Gil-Ortiz; Ignacio Fita; Vicente Rubio
Journal:  Structure       Date:  2002-03       Impact factor: 5.006

2.  Substructure solution with SHELXD.

Authors:  Thomas R Schneider; George M Sheldrick
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-09-28

3.  Effects of naturally occurring osmolytes on protein stability and solubility: issues important in protein crystallization.

Authors:  D W Bolen
Journal:  Methods       Date:  2004-11       Impact factor: 3.608

4.  Rational protein crystallization by mutational surface engineering.

Authors:  Zygmunt S Derewenda
Journal:  Structure       Date:  2004-04       Impact factor: 5.006

5.  Biochemical properties of recombinant human and mouse N-acetylglutamate synthase.

Authors:  Ljubica Caldovic; Giselle Y Lopez; Nantaporn Haskins; Maria Panglao; Dashuang Shi; Hiroki Morizono; Mendel Tuchman
Journal:  Mol Genet Metab       Date:  2006-03       Impact factor: 4.797

6.  Cloning and expression of the human N-acetylglutamate synthase gene.

Authors:  Ljubica Caldovic; Hiroki Morizono; Maria Gracia Panglao; Rene Gallegos; Xiaolin Yu; Dashuang Shi; Michael H Malamy; Norma M Allewell; Mendel Tuchman
Journal:  Biochem Biophys Res Commun       Date:  2002-12-13       Impact factor: 3.575

7.  Null mutations in the N-acetylglutamate synthase gene associated with acute neonatal disease and hyperammonemia.

Authors:  Ljubica Caldovic; Hiroki Morizono; Maria Gracia Panglao; Sabrina F Cheng; Seymour Packman; Mendel Tuchman
Journal:  Hum Genet       Date:  2003-02-20       Impact factor: 4.132

Review 8.  Mammalian N-acetylglutamate synthase.

Authors:  Hiroki Morizono; Ljubica Caldovic; Dashuang Shi; Mendel Tuchman
Journal:  Mol Genet Metab       Date:  2004-04       Impact factor: 4.797

9.  Mutation analysis in patients with N-acetylglutamate synthase deficiency.

Authors:  Johannes Häberle; Eva Schmidt; Silke Pauli; Joachim Gerhard Kreuder; Barbara Plecko; Axel Galler; Benedicht Wermuth; Erik Harms; Hans Georg Koch
Journal:  Hum Mutat       Date:  2003-06       Impact factor: 4.878

Review 10.  N-acetylglutamate and its changing role through evolution.

Authors:  Ljubica Caldovic; Mendel Tuchman
Journal:  Biochem J       Date:  2003-06-01       Impact factor: 3.857
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  7 in total

1.  Structures of the N-acetyltransferase domain of Xylella fastidiosa N-acetyl-L-glutamate synthase/kinase with and without a His tag bound to N-acetyl-L-glutamate.

Authors:  Gengxiang Zhao; Zhongmin Jin; Norma M Allewell; Mendel Tuchman; Dashuang Shi
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-01-01       Impact factor: 1.056

2.  Structure of the complex of Neisseria gonorrhoeae N-acetyl-L-glutamate synthase with a bound bisubstrate analog.

Authors:  Gengxiang Zhao; Norma M Allewell; Mendel Tuchman; Dashuang Shi
Journal:  Biochem Biophys Res Commun       Date:  2012-12-20       Impact factor: 3.575

3.  Replacement of the arginine biosynthesis operon in Xanthomonadales by lateral gene transfer.

Authors:  Wanessa C Lima; Carlos F M Menck
Journal:  J Mol Evol       Date:  2008-02-28       Impact factor: 2.395

4.  The crystal structure of N-acetyl-L-glutamate synthase from Neisseria gonorrhoeae provides insights into mechanisms of catalysis and regulation.

Authors:  Dashuang Shi; Vatsala Sagar; Zhongmin Jin; Xiaolin Yu; Ljubica Caldovic; Hiroki Morizono; Norma M Allewell; Mendel Tuchman
Journal:  J Biol Chem       Date:  2008-01-09       Impact factor: 5.157

5.  A novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.

Authors:  Dashuang Shi; Yongdong Li; Juan Cabrera-Luque; Zhongmin Jin; Xiaolin Yu; Gengxiang Zhao; Nantaporn Haskins; Norma M Allewell; Mendel Tuchman
Journal:  PLoS One       Date:  2011-12-12       Impact factor: 3.240

Review 6.  The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms.

Authors:  Dashuang Shi; Norma M Allewell; Mendel Tuchman
Journal:  Int J Mol Sci       Date:  2015-06-09       Impact factor: 5.923

7.  A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase.

Authors:  Qiuhao Qu; Hiroki Morizono; Dashuang Shi; Mendel Tuchman; Ljubica Caldovic
Journal:  BMC Biochem       Date:  2007-04-10       Impact factor: 4.059
  7 in total

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