Literature DB >> 16954283

Diagnostic value of reverse transcription-PCR for detection of cytomegalovirus pp67 in samples from solid-organ transplant recipients.

Ursula Meyer-Koenig1, Ilona Romberg, Kerstin Schneider, Manfred Weidmann, Winfried V Kern, Frank T Hufert.   

Abstract

We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.

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Year:  2006        PMID： 16954283      PMCID： PMC1594687          DOI： 10.1128/JCM.00493-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  HCMV pp67-mRNA detection by nucleic acid sequence-based amplification in renal transplant patients.

Authors:  C Merlino; M Bergallo; G Gregori; F Sinesi; C Bollero; R Cavallo
Journal:  New Microbiol       Date:  2002-01       Impact factor: 2.479

2.  Cytomegalovirus infection in organ-transplant recipients: diagnostic value of pp65 antigen test, qualitative polymerase chain reaction (PCR) and quantitative Taqman PCR.

Authors:  Ursula Meyer-Koenig; Manfred Weidmann; Günter Kirste; Frank T Hufert
Journal:  Transplantation       Date:  2004-06-15       Impact factor: 4.939

3.  Nucleic acid sequence-based amplification.

Authors:  J Compton
Journal:  Nature       Date:  1991-03-07       Impact factor: 49.962

4.  Diagnostic implications of human cytomegalovirus immediate early-1 and pp67 mRNA detection in whole-blood samples from liver transplant patients using nucleic acid sequence-based amplification.

Authors:  M J Blok; I Lautenschlager; V J Goossens; J M Middeldorp; C Vink; K Höckerstedt; C A Bruggeman
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

5.  Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograft recipients by nucleic acid sequence-based amplification.

Authors:  M J Blok; V J Goossens; S J Vanherle; B Top; N Tacken; J M Middeldorp; M H Christiaans; J P van Hooff; C A Bruggeman
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

6.  Clinical significance of expression of human cytomegalovirus pp67 late transcript in heart, lung, and bone marrow transplant recipients as determined by nucleic acid sequence-based amplification.

Authors:  G Gerna; F Baldanti; J M Middeldorp; M Furione; M Zavattoni; D Lilleri; M G Revello
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

7.  Rapid detection of human pathogenic orthobunyaviruses.

Authors:  Manfred Weidmann; Veronique Rudaz; Marcio R T Nunes; Pedro F C Vasconcelos; Frank T Hufert
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

8.  Rapid detection protocol for filoviruses.

Authors:  Manfred Weidmann; Elke Mühlberger; Frank T Hufert
Journal:  J Clin Virol       Date:  2004-05       Impact factor: 3.168

9.  Prospective evaluation of the clinical utility of different methods for the detection of human cytomegalovirus disease after liver transplantation.

Authors:  Daniel Seehofer; Helga Meisel; Nada Rayes; Angela Stein; Jan M Langrehr; Utz Settmacher; Peter Neuhaus
Journal:  Am J Transplant       Date:  2004-08       Impact factor: 8.086

10.  CMV infection of liver transplant recipients: comparison of antigenemia and molecular biology assays.

Authors:  M L Amorim; J M Cabeda; R Seca; A C Mendes; A P Castro; J M Amorim
Journal:  BMC Infect Dis       Date:  2001-05-22       Impact factor: 3.090
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  1 in total

1.  Reverse transcriptase-polymerase chain reaction to evaluate human cytomegalovirus lytic gene expression.

Authors:  Massimiliano Bergallo; Cristina Costa; Maria Elena Terlizzi; Samuela Margio; Francesca Sidoti; Sara Astegiano; Franca Sinesi; Rossana Cavallo
Journal:  Mol Biotechnol       Date:  2008-05-31       Impact factor: 2.695
  1 in total

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