Manfred Weidmann1, Elke Mühlberger, Frank T Hufert. 1. Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany. Weidmann@ukl.uni-freiburg.de
Abstract
BACKGROUND: The incidence of filovirus disease outbreaks has been increasing in recent years. Although there have been advances in the developments of diagnostics, field tests are rare. Apart from family members of infected patients, health care workers are at high risk of being infected during the initial phase of an outbreak. RT-PCR has been shown to be helpful in containing outbreaks. OBJECTIVES: To develop Taqman-RT-PCR for the detection of Ebola-Zaire virus (EBOV-Z), Ebola-Sudan virus (EBOV-S) and Marburg virus (MBGV). STUDY DESIGN: Quantitative Taqman-RT-PCRs for the detection of these viruses were developed and established on a portable Smartcycler TD. RESULTS AND CONCLUSIONS: All three assays were highly sensitive and specific. The mobility of the assay system may help to contain future outbreaks.
BACKGROUND: The incidence of filovirus disease outbreaks has been increasing in recent years. Although there have been advances in the developments of diagnostics, field tests are rare. Apart from family members of infectedpatients, health care workers are at high risk of being infected during the initial phase of an outbreak. RT-PCR has been shown to be helpful in containing outbreaks. OBJECTIVES: To develop Taqman-RT-PCR for the detection of Ebola-Zaire virus (EBOV-Z), Ebola-Sudan virus (EBOV-S) and Marburg virus (MBGV). STUDY DESIGN: Quantitative Taqman-RT-PCRs for the detection of these viruses were developed and established on a portable Smartcycler TD. RESULTS AND CONCLUSIONS: All three assays were highly sensitive and specific. The mobility of the assay system may help to contain future outbreaks.
Authors: Darci R Smith; Sarah McCarthy; Andrew Chrovian; Gene Olinger; Andrea Stossel; Thomas W Geisbert; Lisa E Hensley; John H Connor Journal: Antiviral Res Date: 2010-05-07 Impact factor: 5.970
Authors: Adrienne R Trombley; Leslie Wachter; Jeffrey Garrison; Valerie A Buckley-Beason; Jordan Jahrling; Lisa E Hensley; Randal J Schoepp; David A Norwood; Augustine Goba; Joseph N Fair; David A Kulesh Journal: Am J Trop Med Hyg Date: 2010-05 Impact factor: 2.345