Literature DB >> 18516703

Reverse transcriptase-polymerase chain reaction to evaluate human cytomegalovirus lytic gene expression.

Massimiliano Bergallo1, Cristina Costa, Maria Elena Terlizzi, Samuela Margio, Francesca Sidoti, Sara Astegiano, Franca Sinesi, Rossana Cavallo.   

Abstract

This paper describes the development of four Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assays devised to evaluate lytic (UL123, immediate-early; UL54, early; UL65, late; and UL99, true late) human cytomegalovirus (HCMV) transcripts. Subsequently, the assays have been validated evaluating the HCMV lytic gene expression in 28 samples (peripheral blood leukocytes, PBLs) from 14 renal transplant recipients. Although the assessment of HCMV transcriptional profile could be useful to evaluate viral reactivation state, further studies on dynamics of viral transcripts in different clinical settings are needed to determine the role of transcriptional profiling in virological monitoring and therapeutic management in immunocompromised patients.

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Year:  2008        PMID： 18516703     DOI： 10.1007/s12033-008-9070-7

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  20 in total

1.  Clinical impact of ganciclovir-resistant cytomegalovirus infections in solid organ transplant patients.

Authors:  G Boivin; N Goyette; C Gilbert; A Humar; E Covington
Journal:  Transpl Infect Dis       Date:  2005 Sep-Dec       Impact factor: 2.228

2.  Quantitation of cytomegalovirus DNA by real-time polymerase chain reaction in peripheral blood specimens of patients with solid organ transplants: comparison with end-point PCR and pp65 antigen test.

Authors:  Tiziano Allice; Marco Enrietto; Fabrizia Pittaluga; Silvia Varetto; Alessandro Franchello; Giovanna Marchiaro; Valeria Ghisetti
Journal:  J Med Virol       Date:  2006-07       Impact factor: 2.327

3.  Diagnostic value of reverse transcription-PCR for detection of cytomegalovirus pp67 in samples from solid-organ transplant recipients.

Authors:  Ursula Meyer-Koenig; Ilona Romberg; Kerstin Schneider; Manfred Weidmann; Winfried V Kern; Frank T Hufert
Journal:  J Clin Microbiol       Date:  2006-09       Impact factor: 5.948

4.  Real-time PCR assay compared with antigenemia assay for detecting cytomegalovirus infection in kidney transplant recipients.

Authors:  D J Kim; S J Kim; J Park; G S Choi; S Lee; C D Kwon; C Ki; J Joh
Journal:  Transplant Proc       Date:  2007-06       Impact factor: 1.066

5.  A polymerase chain reaction to detect a spliced late transcript of human cytomegalovirus in the blood of bone marrow transplant recipients.

Authors:  P N Nelson; B K Rawal; Y S Boriskin; K E Mathers; R L Powles; H M Steel; Y S Tryhorn; P D Butcher; J C Booth
Journal:  J Virol Methods       Date:  1996-02       Impact factor: 2.014

6.  Monitoring of cytomegalovirus infection and disease in bone marrow recipients by reverse transcription-PCR and comparison with PCR and blood and urine cultures.

Authors:  J Gozlan; J P Laporte; S Lesage; M Labopin; A Najman; N C Gorin; J C Petit
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

7.  Early detection of cytomegalovirus (CMV) infection in bone marrow transplant patients by reverse transcription-PCR for CMV spliced late gene UL21.5: a two site evaluation.

Authors:  Yu S Boriskin; K Fuller; R L Powles; I B Vipond; P S Rice; J C Booth; E O Caul; P D Butcher
Journal:  J Clin Virol       Date:  2002-02       Impact factor: 3.168

8.  Evaluation of human cytomegalovirus gene expression in thoracic organ transplant recipients using nucleic acid sequence-based amplification.

Authors:  N Oldenburg; K M Lam; M A Khan; B Top; N M Tacken; A McKie; G W Mikhail; J M Middeldorp; A Wright; N R Banner; M Yacoub
Journal:  Transplantation       Date:  2000-10-27       Impact factor: 4.939

9.  Use of a DNAemia cut-off for monitoring human cytomegalovirus infection reduces the number of preemptively treated children and young adults receiving hematopoietic stem-cell transplantation compared with qualitative pp65 antigenemia.

Authors:  Daniele Lilleri; Giuseppe Gerna; Milena Furione; Maria Ester Bernardo; Giovanna Giorgiani; Stefania Telli; Fausto Baldanti; Franco Locatelli
Journal:  Blood       Date:  2007-06-19       Impact factor: 22.113

10.  Human cytomegalovirus immediate early and late transcripts in peripheral blood leukocytes: diagnostic value in renal transplant recipients.

Authors:  U Meyer-König; A Serr; D von Laer; G Kirste; C Wolff; O Haller; D Neumann-Haefelin; F T Hufert
Journal:  J Infect Dis       Date:  1995-03       Impact factor: 5.226
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  3 in total

1.  An antisense transcript in the human cytomegalovirus UL87 gene region.

Authors:  Yanping Ma; Ning Wang; Mali Li; Shuang Gao; Lin Wang; Yaohua Ji; Ying Qi; Rong He; Zhengrong Sun; Qiang Ruan
Journal:  Virol J       Date:  2011-11-11       Impact factor: 4.099

Review 2.  Functional annotation of human cytomegalovirus gene products: an update.

Authors:  Ellen Van Damme; Marnix Van Loock
Journal:  Front Microbiol       Date:  2014-05-19       Impact factor: 5.640

3.  A mouse model of interstitial pneumonitis induced by murine cytomegalovirus infection after allogeneic skin transplantation.

Authors:  Dequn Ni; Haiyang Yu; Wei Zhang; Lin Gan; Jiqiang Zhao; Mingli Wang; Jason Chen
Journal:  Biomed Res Int       Date:  2013-07-02       Impact factor: 3.411
  3 in total

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