Literature DB >> 16757640

Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1.

D R McClernon1, C Vavro, M St Clair.   

Abstract

We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.

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Year:  2006        PMID： 16757640      PMCID： PMC1489398          DOI： 10.1128/JCM.02187-05

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

Review 1.  Characteristics and applications of nucleic acid sequence-based amplification (NASBA).

Authors:  Birgit Deiman; Pierre van Aarle; Peter Sillekens
Journal:  Mol Biotechnol       Date:  2002-02       Impact factor: 2.695

2.  Evaluation of the NucliSens EasyQ assay in HIV-1-infected individuals in South Africa.

Authors:  W Stevens; T Wiggill; P Horsfield; L Coetzee; L E Scott
Journal:  J Virol Methods       Date:  2004-12-15       Impact factor: 2.014

3.  Multicenter evaluation of the NucliSens EasyQ HIV-1 v1.1 assay for the quantitative detection of HIV-1 RNA in plasma.

Authors:  C de Mendoza; M Koppelman; B Montès; V Ferre; V Soriano; H Cuypers; M Segondy; T Oosterlaken
Journal:  J Virol Methods       Date:  2005-04-25       Impact factor: 2.014

4.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

5.  Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay.

Authors:  Jun Yao; Zhen Liu; Lung-Sang Ko; Gang Pan; Yan Jiang
Journal:  J Virol Methods       Date:  2005-10       Impact factor: 2.014

6.  Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma.

Authors:  D G Murphy; L Côté; M Fauvel; P René; J Vincelette
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

Review 7.  Impact of clade diversity on HIV-1 virulence, antiretroviral drug sensitivity and drug resistance.

Authors:  Shalom Spira; Mark A Wainberg; Hugues Loemba; Dan Turner; Bluma G Brenner
Journal:  J Antimicrob Chemother       Date:  2003-02       Impact factor: 5.790
  7 in total
  7 in total

Review 1.  Advantages of peptide nucleic acids as diagnostic platforms for detection of nucleic acids in resource-limited settings.

Authors:  Ning Zhang; Daniel H Appella
Journal:  J Infect Dis       Date:  2010-04-15       Impact factor: 5.226

2.  Magnetic silica extraction for low-viremia human immunodeficiency virus type 1 genotyping.

Authors:  D R McClernon; E Ramsey; M St Clair
Journal:  J Clin Microbiol       Date:  2006-11-22       Impact factor: 5.948

3.  Detection of Aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real-time nucleic acid sequence-based amplification.

Authors:  Yanan Zhao; Steven Park; Peter Warn; Raghdaa Shrief; Elizabeth Harrison; David S Perlin
Journal:  J Clin Microbiol       Date:  2010-02-03       Impact factor: 5.948

4.  Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections.

Authors:  Yanan Zhao; Steven Park; Barry N Kreiswirth; Christine C Ginocchio; Raphaël Veyret; Ali Laayoun; Alain Troesch; David S Perlin
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

5.  Molecular beacons in diagnostics.

Authors:  Sanjay Tyagi; Fred Russell Kramer
Journal:  F1000 Med Rep       Date:  2012-05-02

6.  Development of a quantitative real-time nucleic acid sequence-based amplification assay with an internal control using molecular beacon probes for selective and sensitive detection of human rhinovirus serotypes.

Authors:  Francesca Sidoti; Massimiliano Bergallo; Maria Elena Terlizzi; Elsa Piasentin Alessio; Sara Astegiano; Giorgio Gasparini; Rossana Cavallo
Journal:  Mol Biotechnol       Date:  2012-03       Impact factor: 2.695

7.  Comparison of the real-time nucleic acid sequence-based amplification (RTi-NASBA) with conventional NASBA, and galactomannan assay for the diagnosis of invasive aspergillosis.

Authors:  Jin Hong Yoo; Su Mi Choi; Dong Gun Lee; Sun Hee Park; Jung Hyun Choi; Eun Young Kwon; Wan Shik Shin
Journal:  J Korean Med Sci       Date:  2007-08       Impact factor: 2.153
  7 in total

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