OBJECTIVE: To investigate the prognostic value for loss of heterozygosity (LOH) of chromosomes 4 and 14q in early-stage colorectal cancer (CRC). METHODS: A total of 70, largely microsatellite stable, tumours and their corresponding normal mucosa were subjected to microdissection and analysed for LOH at chromosomes 4 and 14q by using 13 highly polymorphic microsatellite markers. LOH was correlated with the survival of the patients, using univariate, multivariate and Kaplan-Meier's survival curves. RESULT: LOH at D4S2935, D4S1579 and D4S1595 on chromosome 4 was significantly associated with metastatic recurrence of early-stage CRC. For chromosome arm 14q, two minimal regions of deletion were associated with metastatic recurrence and mapped to neighbouring markers D14S275/D14S49 at 14q12-13 and D14S65/D14S250 at 14q32. High-level loss (loss of five to eight of the informative microsatellite markers) on both chromosomes 4 and 14q, to be an independent prognostic indicator in early-stage CRC was shown by multivariate analysis. CONCLUSION: Determining the LOH of chromosomes 4 and 14q and their extent in primary tumours of patients with early-stage CRC may constitute a molecular signature of metastatic recurrence. This may be achieved if new finding sheds light on the treatment of this subgroup of patients that have been largely ignored.
OBJECTIVE: To investigate the prognostic value for loss of heterozygosity (LOH) of chromosomes 4 and 14q in early-stage colorectal cancer (CRC). METHODS: A total of 70, largely microsatellite stable, tumours and their corresponding normal mucosa were subjected to microdissection and analysed for LOH at chromosomes 4 and 14q by using 13 highly polymorphic microsatellite markers. LOH was correlated with the survival of the patients, using univariate, multivariate and Kaplan-Meier's survival curves. RESULT: LOH at D4S2935, D4S1579 and D4S1595 on chromosome 4 was significantly associated with metastatic recurrence of early-stage CRC. For chromosome arm 14q, two minimal regions of deletion were associated with metastatic recurrence and mapped to neighbouring markers D14S275/D14S49 at 14q12-13 and D14S65/D14S250 at 14q32. High-level loss (loss of five to eight of the informative microsatellite markers) on both chromosomes 4 and 14q, to be an independent prognostic indicator in early-stage CRC was shown by multivariate analysis. CONCLUSION: Determining the LOH of chromosomes 4 and 14q and their extent in primary tumours of patients with early-stage CRC may constitute a molecular signature of metastatic recurrence. This may be achieved if new finding sheds light on the treatment of this subgroup of patients that have been largely ignored.
Authors: R A Risques; V Moreno; E Marcuello; J Petriz; J A Cancelas; F J Sancho; A Torregrosa; G Capella; M A Peinado Journal: Lab Invest Date: 2001-03 Impact factor: 5.662
Authors: Yixin Wang; Tim Jatkoe; Yi Zhang; Matthew G Mutch; Dmitri Talantov; John Jiang; Howard L McLeod; David Atkins Journal: J Clin Oncol Date: 2004-03-29 Impact factor: 44.544
Authors: José María Sayagués; Celia Fontanillo; María del Mar Abad; María González-González; María Eugenia Sarasquete; Maria del Carmen Chillon; Eva Garcia; Oscar Bengoechea; Emilio Fonseca; Marcos Gonzalez-Diaz; Javier De las Rivas; Luís Muñoz-Bellvis; Alberto Orfao Journal: PLoS One Date: 2010-10-29 Impact factor: 3.240
Authors: Michal Sheffer; Manny D Bacolod; Or Zuk; Sarah F Giardina; Hanna Pincas; Francis Barany; Philip B Paty; William L Gerald; Daniel A Notterman; Eytan Domany Journal: Proc Natl Acad Sci U S A Date: 2009-04-09 Impact factor: 11.205
Authors: R P M Brosens; E J T H Belt; J C Haan; T E Buffart; B Carvalho; H Grabsch; P Quirke; M A Cuesta; A F Engel; B Ylstra; G A Meijer Journal: Cell Oncol (Dordr) Date: 2011-06-30 Impact factor: 6.730
Authors: Hisham Eldai; Sathish Periyasamy; Saeed Al Qarni; Maha Al Rodayyan; Sabeena Muhammed Mustafa; Ahmad Deeb; Ebthehal Al Sheikh; Mohammed Afzal; Mohammed Afzal Khan; Mishal Johani; Zeyad Yousef; Mohammad Azhar Aziz Journal: PLoS One Date: 2013-10-30 Impact factor: 3.240
Authors: Jennifer F Knight; Vanessa Y C Sung; Elena Kuzmin; Amber L Couzens; Danielle A de Verteuil; Colin D H Ratcliffe; Paula P Coelho; Radia M Johnson; Payman Samavarchi-Tehrani; Tina Gruosso; Harvey W Smith; Wontae Lee; Sadiq M Saleh; Dongmei Zuo; Hong Zhao; Marie-Christine Guiot; Ryan R Davis; Jeffrey P Gregg; Christopher Moraes; Anne-Claude Gingras; Morag Park Journal: Cell Rep Date: 2018-03-20 Impact factor: 9.423
Authors: Naveed Ishaque; Mohammed L Abba; Christine Hauser; Nitin Patil; Nagarajan Paramasivam; Daniel Huebschmann; Jörg Hendrik Leupold; Gnana Prakash Balasubramanian; Kortine Kleinheinz; Umut H Toprak; Barbara Hutter; Axel Benner; Anna Shavinskaya; Chan Zhou; Zuguang Gu; Jules Kerssemakers; Alexander Marx; Marcin Moniuszko; Miroslaw Kozlowski; Joanna Reszec; Jacek Niklinski; Jürgen Eils; Matthias Schlesner; Roland Eils; Benedikt Brors; Heike Allgayer Journal: Nat Commun Date: 2018-11-14 Impact factor: 14.919