Literature DB >> 16407165

Essential genes of a minimal bacterium.

John I Glass1, Nacyra Assad-Garcia, Nina Alperovich, Shibu Yooseph, Matthew R Lewis, Mahir Maruf, Clyde A Hutchison, Hamilton O Smith, J Craig Venter.   

Abstract

Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28% of the essential protein-coding genes set. Disruption of some genes accelerated M. genitalium growth.

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Year:  2006        PMID： 16407165      PMCID： PMC1324956          DOI： 10.1073/pnas.0510013103

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

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Authors:  K A Reich
Journal:  Res Microbiol       Date:  2000-06       Impact factor: 3.992

2.  Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA.

Authors:  Y Ji; B Zhang; S F Van; P Warren; G Woodnutt; M K Burnham; M Rosenberg
Journal:  Science       Date:  2001-09-21       Impact factor: 47.728

3.  Comprehensive identification of conditionally essential genes in mycobacteria.

Authors:  C M Sassetti; D H Boyd; E J Rubin
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-16       Impact factor: 11.205

4.  Essential Bacillus subtilis genes.

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Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-07       Impact factor: 11.205

5.  Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli.

Authors:  Christopher D Herring; Jeremy D Glasner; Frederick R Blattner
Journal:  Gene       Date:  2003-06-05       Impact factor: 3.688

6.  Global transposon mutagenesis and essential gene analysis of Helicobacter pylori.

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7.  Global transposon mutagenesis and a minimal Mycoplasma genome.

Authors:  C A Hutchison; S N Peterson; S R Gill; R T Cline; O White; C M Fraser; H O Smith; J C Venter
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8.  Suspected utility of enzymes with multiple activities in the small genome Mycoplasma species: the replacement of the missing "household" nucleoside diphosphate kinase gene and activity by glycolytic kinases.

Authors:  J Dennis Pollack; Melissa A Myers; Thomas Dandekar; Richard Herrmann
Journal:  OMICS       Date:  2002

9.  Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis.

Authors:  K Dybvig; C T French; L L Voelker
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

10.  A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.

Authors:  R Allyn Forsyth; Robert J Haselbeck; Kari L Ohlsen; Robert T Yamamoto; Howard Xu; John D Trawick; Daniel Wall; Liangsu Wang; Vickie Brown-Driver; Jamie M Froelich; Kedar G C; Paula King; Melissa McCarthy; Cheryl Malone; Brian Misiner; David Robbins; Zehui Tan; Zhan-yang Zhu Zy; Grant Carr; Deborah A Mosca; Carlos Zamudio; J Gordon Foulkes; Judith W Zyskind
Journal:  Mol Microbiol       Date:  2002-03       Impact factor: 3.501
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  331 in total

Review 1.  Extreme genome reduction in symbiotic bacteria.

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Journal:  Nat Rev Microbiol       Date:  2011-11-08       Impact factor: 60.633

2.  Targeted chromosomal knockouts in Mycoplasma pneumoniae.

Authors:  Radha Krishnakumar; Nacyra Assad-Garcia; Gwynedd A Benders; Quang Phan; Michael G Montague; John I Glass
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

3.  A synthetic DNA transplant.

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Review 4.  How to make a minimal genome for synthetic minimal cell.

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Journal:  Protein Cell       Date:  2010-06-04       Impact factor: 14.870

5.  Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: potential target against mycoplasma infections.

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Journal:  J Biol Chem       Date:  2011-08-11       Impact factor: 5.157

6.  Fewer essential genes in mycoplasmas than previous studies suggest.

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7.  c-di-AMP secreted by intracellular Listeria monocytogenes activates a host type I interferon response.

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8.  The essential gene set of a photosynthetic organism.

Authors:  Benjamin E Rubin; Kelly M Wetmore; Morgan N Price; Spencer Diamond; Ryan K Shultzaberger; Laura C Lowe; Genevieve Curtin; Adam P Arkin; Adam Deutschbauer; Susan S Golden
Journal:  Proc Natl Acad Sci U S A       Date:  2015-10-27       Impact factor: 11.205

9.  Structural insights into the extracytoplasmic thiamine-binding lipoprotein p37 of Mycoplasma hyorhinis.

Authors:  Katherine H Sippel; Arthur H Robbins; Robbie Reutzel; Susan K Boehlein; Kazunori Namiki; Steve Goodison; Mavis Agbandje-McKenna; Charles J Rosser; Robert McKenna
Journal:  J Bacteriol       Date:  2009-02-20       Impact factor: 3.490

10.  Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium.

Authors:  Oxana Musatovova; Subramanian Dhandayuthapani; Joel B Baseman
Journal:  J Bacteriol       Date:  2006-04       Impact factor: 3.490
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