Literature DB >> 11567142

Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA.

Y Ji1, B Zhang, S F Van, P Warren, G Woodnutt, M K Burnham, M Rosenberg.   

Abstract

Comprehensive genomic analysis of the important human pathogen Staphylococcus aureus was achieved by a strategy involving antisense technology in a regulatable gene expression system. In addition to known essential genes, many genes of unknown or poorly defined biological function were identified. This methodology allowed gene function to be characterized in a comprehensive, defined set of conditionally growth-defective/lethal isogenic strains. Quantitative titration of the conditional growth effect was performed either in bacterial culture or in an animal model of infection. This genomic strategy offers an approach to the identification of staphylococcal gene products that could serve as targets for antibiotic discovery.

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Year:  2001        PMID： 11567142     DOI： 10.1126/science.1063566

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  160 in total

1.  The YSIRK-G/S motif of staphylococcal protein A and its role in efficiency of signal peptide processing.

Authors:  Taeok Bae; Olaf Schneewind
Journal:  J Bacteriol       Date:  2003-05       Impact factor: 3.490

2.  Essential Bacillus subtilis genes.

Authors:  K Kobayashi; S D Ehrlich; A Albertini; G Amati; K K Andersen; M Arnaud; K Asai; S Ashikaga; S Aymerich; P Bessieres; F Boland; S C Brignell; S Bron; K Bunai; J Chapuis; L C Christiansen; A Danchin; M Débarbouille; E Dervyn; E Deuerling; K Devine; S K Devine; O Dreesen; J Errington; S Fillinger; S J Foster; Y Fujita; A Galizzi; R Gardan; C Eschevins; T Fukushima; K Haga; C R Harwood; M Hecker; D Hosoya; M F Hullo; H Kakeshita; D Karamata; Y Kasahara; F Kawamura; K Koga; P Koski; R Kuwana; D Imamura; M Ishimaru; S Ishikawa; I Ishio; D Le Coq; A Masson; C Mauël; R Meima; R P Mellado; A Moir; S Moriya; E Nagakawa; H Nanamiya; S Nakai; P Nygaard; M Ogura; T Ohanan; M O'Reilly; M O'Rourke; Z Pragai; H M Pooley; G Rapoport; J P Rawlins; L A Rivas; C Rivolta; A Sadaie; Y Sadaie; M Sarvas; T Sato; H H Saxild; E Scanlan; W Schumann; J F M L Seegers; J Sekiguchi; A Sekowska; S J Séror; M Simon; P Stragier; R Studer; H Takamatsu; T Tanaka; M Takeuchi; H B Thomaides; V Vagner; J M van Dijl; K Watabe; A Wipat; H Yamamoto; M Yamamoto; Y Yamamoto; K Yamane; K Yata; K Yoshida; H Yoshikawa; U Zuber; N Ogasawara
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-07       Impact factor: 11.205

3.  Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.

Authors:  Jane A Thanassi; Sandra L Hartman-Neumann; Thomas J Dougherty; Brian A Dougherty; Michael J Pucci
Journal:  Nucleic Acids Res       Date:  2002-07-15       Impact factor: 16.971

4.  Identification of Listeria monocytogenes genes involved in salt and alkaline-pH tolerance.

Authors:  Rozenn Gardan; Pascale Cossart; Jean Labadie
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

5.  DEG: a database of essential genes.

Authors:  Ren Zhang; Hong-Yu Ou; Chun-Ting Zhang
Journal:  Nucleic Acids Res       Date:  2004-01-01       Impact factor: 16.971

6.  Identification of antibiotic stress-inducible promoters: a systematic approach to novel pathway-specific reporter assays for antibacterial drug discovery.

Authors:  Hans Peter Fischer; Nina A Brunner; Bernd Wieland; Jesse Paquette; Ludwig Macko; Karl Ziegelbauer; Christoph Freiberg
Journal:  Genome Res       Date:  2004-01       Impact factor: 9.043

Review 7.  Determination of the core of a minimal bacterial gene set.

Authors:  Rosario Gil; Francisco J Silva; Juli Peretó; Andrés Moya
Journal:  Microbiol Mol Biol Rev       Date:  2004-09       Impact factor: 11.056

8.  A new code for life.

Authors:  Andrea Rinaldi
Journal:  EMBO Rep       Date:  2004-04       Impact factor: 8.807

9.  Staphylococcus aureus TargetArray: comprehensive differential essential gene expression as a mechanistic tool to profile antibacterials.

Authors:  H Howard Xu; John D Trawick; Robert J Haselbeck; R Allyn Forsyth; Robert T Yamamoto; Rich Archer; Joe Patterson; Molly Allen; Jamie M Froelich; Ian Taylor; Danny Nakaji; Randy Maile; G C Kedar; Marshall Pilcher; Vickie Brown-Driver; Melissa McCarthy; Amy Files; David Robbins; Paula King; Susan Sillaots; Cheryl Malone; Carlos S Zamudio; Terry Roemer; Liangsu Wang; Philip J Youngman; Daniel Wall
Journal:  Antimicrob Agents Chemother       Date:  2010-06-14       Impact factor: 5.191

Review 10.  How to make a minimal genome for synthetic minimal cell.

Authors:  Liu-Yan Zhang; Su-Hua Chang; Jing Wang
Journal:  Protein Cell       Date:  2010-06-04       Impact factor: 14.870
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