Literature DB >> 20722737

Fewer essential genes in mycoplasmas than previous studies suggest.

Kevin Dybvig1, Ping Lao, David S Jordan, Warren L Simmons.   

Abstract

Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but essential in Bacillus subtilis.
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

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Year:  2010        PMID： 20722737      PMCID： PMC2974803          DOI： 10.1111/j.1574-6968.2010.02078.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  18 in total

1.  Gene transfer in Mycoplasma pulmonis.

Authors:  Amy M Teachman; C Todd French; Huilan Yu; Warren L Simmons; Kevin Dybvig
Journal:  J Bacteriol       Date:  2002-02       Impact factor: 3.490

2.  Essential Bacillus subtilis genes.

Authors:  K Kobayashi; S D Ehrlich; A Albertini; G Amati; K K Andersen; M Arnaud; K Asai; S Ashikaga; S Aymerich; P Bessieres; F Boland; S C Brignell; S Bron; K Bunai; J Chapuis; L C Christiansen; A Danchin; M Débarbouille; E Dervyn; E Deuerling; K Devine; S K Devine; O Dreesen; J Errington; S Fillinger; S J Foster; Y Fujita; A Galizzi; R Gardan; C Eschevins; T Fukushima; K Haga; C R Harwood; M Hecker; D Hosoya; M F Hullo; H Kakeshita; D Karamata; Y Kasahara; F Kawamura; K Koga; P Koski; R Kuwana; D Imamura; M Ishimaru; S Ishikawa; I Ishio; D Le Coq; A Masson; C Mauël; R Meima; R P Mellado; A Moir; S Moriya; E Nagakawa; H Nanamiya; S Nakai; P Nygaard; M Ogura; T Ohanan; M O'Reilly; M O'Rourke; Z Pragai; H M Pooley; G Rapoport; J P Rawlins; L A Rivas; C Rivolta; A Sadaie; Y Sadaie; M Sarvas; T Sato; H H Saxild; E Scanlan; W Schumann; J F M L Seegers; J Sekiguchi; A Sekowska; S J Séror; M Simon; P Stragier; R Studer; H Takamatsu; T Tanaka; M Takeuchi; H B Thomaides; V Vagner; J M van Dijl; K Watabe; A Wipat; H Yamamoto; M Yamamoto; Y Yamamoto; K Yamane; K Yata; K Yoshida; H Yoshikawa; U Zuber; N Ogasawara
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-07       Impact factor: 11.205

3.  The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis.

Authors:  I Chambaud; R Heilig; S Ferris; V Barbe; D Samson; F Galisson; I Moszer; K Dybvig; H Wróblewski; A Viari; E P Rocha; A Blanchard
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

4.  Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein.

Authors:  Judita Mascarenhas; Jörg Soppa; Alexander V Strunnikov; Peter L Graumann
Journal:  EMBO J       Date:  2002-06-17       Impact factor: 11.598

5.  High-frequency variation in Mycoplasma pulmonis colony size.

Authors:  K Dybvig; J W Simecka; H L Watson; G H Cassell
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

6.  Identification and characterization of IS1138, a transposable element from Mycoplasma pulmonis that belongs to the IS3 family.

Authors:  B Bhugra; K Dybvig
Journal:  Mol Microbiol       Date:  1993-02       Impact factor: 3.501

7.  Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis.

Authors:  K Dybvig; C T French; L L Voelker
Journal:  J Bacteriol       Date:  2000-08       Impact factor: 3.490

8.  Experimental Mycoplasma pulmonis infection in pathogen-free mice. Models for studying mycoplasmosis of the respiratory tract.

Authors:  J R Lindsey; H Cassell
Journal:  Am J Pathol       Date:  1973-07       Impact factor: 4.307

9.  The Vsa proteins modulate susceptibility of Mycoplasma pulmonis to complement killing, hemadsorption, and adherence to polystyrene.

Authors:  Warren L Simmons; Kevin Dybvig
Journal:  Infect Immun       Date:  2003-10       Impact factor: 3.441

10.  Development of an aerosol model of murine respiratory mycoplasmosis in mice.

Authors:  J K Davis; R B Thorp; R F Parker; H White; D Dziedzic; J D'Arcy; G H Cassell
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441
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  12 in total

1.  Non-essential ribosomal proteins in bacteria and archaea identified using COGs.

Authors:  Michael Y Galperin; Yuri I Wolf; Sofya K Garushyants; Roberto Vera Alvarez; Eugene V Koonin
Journal:  J Bacteriol       Date:  2021-03-22       Impact factor: 3.490

2.  Mycoplasma pulmonis Vsa proteins and polysaccharide modulate adherence to pulmonary epithelial cells.

Authors:  Jeffrey R Bolland; Kevin Dybvig
Journal:  FEMS Microbiol Lett       Date:  2012-04-10       Impact factor: 2.742

3.  The Mycoplasma gallisepticum virulence factor lipoprotein MslA is a novel polynucleotide binding protein.

Authors:  Yumiko Masukagami; Kelly A Tivendale; Karim Mardani; Idan Ben-Barak; Philip F Markham; Glenn F Browning
Journal:  Infect Immun       Date:  2013-06-24       Impact factor: 3.441

4.  The Vsa shield of Mycoplasma pulmonis is antiphagocytic.

Authors:  Brandon M Shaw; Warren L Simmons; Kevin Dybvig
Journal:  Infect Immun       Date:  2011-11-14       Impact factor: 3.441

5.  Rhamnose biosynthesis in mycoplasmas requires precursor glycans larger than monosaccharide.

Authors:  David S Jordan; James M Daubenspeck; Kevin Dybvig
Journal:  Mol Microbiol       Date:  2013-07-19       Impact factor: 3.501

6.  Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.

Authors:  Patrick C Thiaville; Basma El Yacoubi; Caroline Köhrer; Jennifer J Thiaville; Chris Deutsch; Dirk Iwata-Reuyl; Jo Marie Bacusmo; Jean Armengaud; Yoshitaka Bessho; Collin Wetzel; Xiaoyu Cao; Patrick A Limbach; Uttam L RajBhandary; Valérie de Crécy-Lagard
Journal:  Mol Microbiol       Date:  2015-10-07       Impact factor: 3.501

7.  Specific evolution of F1-like ATPases in mycoplasmas.

Authors:  Laure Béven; Claire Charenton; Alain Dautant; Guillaume Bouyssou; Fabien Labroussaa; Anna Sköllermo; Anja Persson; Alain Blanchard; Pascal Sirand-Pugnet
Journal:  PLoS One       Date:  2012-06-07       Impact factor: 3.240

8.  General N-and O-Linked Glycosylation of Lipoproteins in Mycoplasmas and Role of Exogenous Oligosaccharide.

Authors:  James M Daubenspeck; David S Jordan; Warren Simmons; Matthew B Renfrow; Kevin Dybvig
Journal:  PLoS One       Date:  2015-11-23       Impact factor: 3.240

9.  Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.

Authors:  Shukriti Sharma; Philip F Markham; Glenn F Browning
Journal:  PLoS One       Date:  2014-06-04       Impact factor: 3.240

10.  Predicting the minimal translation apparatus: lessons from the reductive evolution of mollicutes.

Authors:  Henri Grosjean; Marc Breton; Pascal Sirand-Pugnet; Florence Tardy; François Thiaucourt; Christine Citti; Aurélien Barré; Satoko Yoshizawa; Dominique Fourmy; Valérie de Crécy-Lagard; Alain Blanchard
Journal:  PLoS Genet       Date:  2014-05-08       Impact factor: 5.917
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