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Literature DB >> 16348974

Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils.

C C Young¹, R L Burghoff, L G Keim, V Minak-Bernero, J R Lute, S M Hinton.

Abstract

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids.

Entities: Chemical Disease

Year: 1993 PMID： 16348974 PMCID： PMC182198 DOI： 10.1128/aem.59.6.1972-1974.1993

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

6 in total

1. An efficient method for isolation of RNA and DNA from plants containing polyphenolics.

Authors: M E John
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

2. DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors: William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal: Appl Environ Microbiol Date: 1988-03 Impact factor: 4.792

3. Poliovirus retention in soil columns after application of chemical- and polyelectrolyte-conditioned dewatered sludges.

Authors:
Journal: Appl Environ Microbiol Date: 1988-04 Impact factor: 4.792

4. Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.

Authors: Y L Tsai; B H Olson
Journal: Appl Environ Microbiol Date: 1992-02 Impact factor: 4.792

5. Rapid method for direct extraction of DNA from soil and sediments.

Authors: Y L Tsai; B H Olson
Journal: Appl Environ Microbiol Date: 1991-04 Impact factor: 4.792

6. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.

Authors: D J Lane; B Pace; G J Olsen; D A Stahl; M L Sogin; N R Pace
Journal: Proc Natl Acad Sci U S A Date: 1985-10 Impact factor: 11.205

6 in total

36 in total

1. Genus- and species-specific PCR-based detection of dairy propionibacteria in environmental samples by using primers targeted to the genes encoding 16S rRNA.

Authors: F Rossi; S Torriani; F Dellaglio
Journal: Appl Environ Microbiol Date: 1999-09 Impact factor: 4.792

2. The presence of humic substances and DNA in RNA extracts affects hybridization results.

Authors: E W Alm; D Zheng; L Raskin
Journal: Appl Environ Microbiol Date: 2000-10 Impact factor: 4.792

3. Use of randomly amplified polymorphic DNA as a means of developing genus- and strain-specific Streptomyces DNA probes.

Authors: M A Roberts; D L Crawford
Journal: Appl Environ Microbiol Date: 2000-06 Impact factor: 4.792

4. Detection of Mycobacterium ulcerans in environmental samples during an outbreak of ulcerative disease.

Authors: B C Ross; P D Johnson; F Oppedisano; L Marino; A Sievers; T Stinear; J A Hayman; M G Veitch; R M Robins-Browne
Journal: Appl Environ Microbiol Date: 1997-10 Impact factor: 4.792

5. Fine-scale genetic diversity among Burkholderia pseudomallei soil isolates in northeast Thailand.

Authors: Jana M U'ren; Heidie Hornstra; Talima Pearson; James M Schupp; Benjamin Leadem; Shalamar Georgia; Rasana W Sermswan; Paul Keim
Journal: Appl Environ Microbiol Date: 2007-08-24 Impact factor: 4.792

6. Characterization of a defined 2,3,5, 6-tetrachlorobiphenyl-ortho-dechlorinating microbial community by comparative sequence analysis of genes coding for 16S rRNA.

Authors: T R Holoman; M A Elberson; L A Cutter; H D May; K R Sowers
Journal: Appl Environ Microbiol Date: 1998-09 Impact factor: 4.792

7. Analysis of subfossil molecular remains of purple sulfur bacteria in a lake sediment.

Authors: M J Coolen; J Overmann
Journal: Appl Environ Microbiol Date: 1998-11 Impact factor: 4.792

8. Influence of pretreatment and experimental conditions on electrophoretic mobility and hydrophobicity of Cryptosporidium parvum oocysts.

Authors: C F Brush; M F Walter; L J Anguish; W C Ghiorse
Journal: Appl Environ Microbiol Date: 1998-11 Impact factor: 4.792

9. Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method.

Authors: K J Purdy; T M Embley; S Takii; D B Nedwell
Journal: Appl Environ Microbiol Date: 1996-10 Impact factor: 4.792

10. A simple, efficient method for the separation of humic substances and DNA from environmental samples.

Authors: C R Jackson; J P Harper; D Willoughby; E E Roden; P F Churchill
Journal: Appl Environ Microbiol Date: 1997-12 Impact factor: 4.792