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Literature DB >> 16535760

A simple, efficient method for the separation of humic substances and DNA from environmental samples.

C R Jackson, J P Harper, D Willoughby, E E Roden, P F Churchill.

Abstract

Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.

Entities: Chemical Species

Year: 1997 PMID： 16535760 PMCID： PMC1389316 DOI： 10.1128/aem.63.12.4993-4995.1997

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

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