Literature DB >> 16535431

Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method.

K J Purdy, T M Embley, S Takii, D B Nedwell.   

Abstract

We describe a novel hydroxyapatite spin-column method of nucleic acid extraction from natural sediments by which DNA and rRNA can be extracted separately. This very rapid method produces pure nucleic acid that can be utilized in some of the most common molecular biological procedures used in the analysis of natural microbial communities.

Entities:  

Year:  1996        PMID： 16535431      PMCID： PMC1388969          DOI： 10.1128/aem.62.10.3905-3907.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors:  William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

2.  Rapid method for direct extraction of DNA from soil and sediments.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

3.  Direct extraction and purification of rRNA for ecological studies.

Authors:  M A Moran; V L Torsvik; T Torsvik; R E Hodson
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

4.  The basis of fractionation of single-stranded nucleic acids on hydroxylapatite.

Authors:  H G Martinson
Journal:  Biochemistry       Date:  1973-07-03       Impact factor: 3.162

5.  Role of the double-stranded nucleic acid backbone configuration in adsorption interactions.

Authors:  H G Martinson
Journal:  Biochemistry       Date:  1973-07-03       Impact factor: 3.162

6.  Chromatography of nucleic acids on hydroxyapatite.

Authors:  G Bernardi
Journal:  Nature       Date:  1965-05-22       Impact factor: 49.962

7.  Comparison of methods of DNA extraction from stream sediments.

Authors:  L G Leff; J R Dana; J V McArthur; L J Shimkets
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

8.  Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast.

Authors:  C C Tebbe; W Vahjen
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

9.  Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment.

Authors:  M I Moré; J B Herrick; M C Silva; W C Ghiorse; E L Madsen
Journal:  Appl Environ Microbiol       Date:  1994-05       Impact factor: 4.792

10.  Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

Authors:  D A Stahl; B Flesher; H R Mansfield; L Montgomery
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792
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  31 in total

1.  Simultaneous recovery of RNA and DNA from soils and sediments.

Authors:  R A Hurt; X Qiu; L Wu; Y Roh; A V Palumbo; J M Tiedje; J Zhou
Journal:  Appl Environ Microbiol       Date:  2001-10       Impact factor: 4.792

2.  The Structure of Microbial Communities in Soil and the Lasting Impact of Cultivation.

Authors:  D.H. Buckley; T.M. Schmidt
Journal:  Microb Ecol       Date:  2001-07       Impact factor: 4.552

3.  Parallel characterization of anaerobic toluene- and ethylbenzene-degrading microbial consortia by PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization, and DNA microarray technology.

Authors:  Yoshikazu Koizumi; John J Kelly; Tatsunori Nakagawa; Hidetoshi Urakawa; Saïd El-Fantroussi; Saleh Al-Muzaini; Manabu Fukui; Yoshikuni Urushigawa; David A Stahl
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

4.  Dominant microbial composition and its vertical distribution in saline meromictic Lake Kaiike (Japan) as revealed by quantitative oligonucleotide probe membrane hybridization.

Authors:  Yoshikazu Koizumi; Hisaya Kojima; Manabu Fukui
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

5.  Contrasting patterns of niche partitioning between two anaerobic terminal oxidizers of organic matter.

Authors:  Brian B Oakley; Franck Carbonero; Scot E Dowd; Robert J Hawkins; Kevin J Purdy
Journal:  ISME J       Date:  2011-11-24       Impact factor: 10.302

6.  Genotypic distribution of a specialist model microorganism, Methanosaeta, along an estuarine gradient: does metabolic restriction limit niche differentiation potential?

Authors:  Franck Carbonero; Brian B Oakley; Robert J Hawkins; Kevin J Purdy
Journal:  Microb Ecol       Date:  2011-12-20       Impact factor: 4.552

7.  Impacts of inter- and intralaboratory variations on the reproducibility of microbial community analyses.

Authors:  Yao Pan; Levente Bodrossy; Peter Frenzel; Anne-Grethe Hestnes; Sascha Krause; Claudia Lüke; Marion Meima-Franke; Henri Siljanen; Mette M Svenning; Paul L E Bodelier
Journal:  Appl Environ Microbiol       Date:  2010-09-24       Impact factor: 4.792

8.  Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques.

Authors:  Hideyuki Tamaki; Yuji Sekiguchi; Satoshi Hanada; Kazunori Nakamura; Nakao Nomura; Masatoshi Matsumura; Yoichi Kamagata
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

9.  A molecular approach to identify active microbes in environmental eukaryote clone libraries.

Authors:  Thorsten Stoeck; Alexandra Zuendorf; Hans-Werner Breiner; Anke Behnke
Journal:  Microb Ecol       Date:  2007-02       Impact factor: 4.552

10.  Comparison of Euryarchaea strains in the guts and food-soil of the soil-feeding termite Cubitermes fungifaber across different soil types.

Authors:  S E Donovan; K J Purdy; M D Kane; P Eggleton
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792
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