High Survivability of Cheese Whey-Grown Rhizobium meliloti Cells upon Exposure to Physical Stress.

Whey, a by-product of the dairy industry, has been found to protect the rhizobia cells during freezing and thawing. Cells of rhizobia grown on whey sustained freezing better at -18 degrees C than did cells grown on mannitol or sucrose. Suspensions of cells grown on whey or mannitol that were suspended in whey performed equally well at -18 and -80 degrees C, with 94 and 100% survival, respectively. Whey-grown rhizobia in pellets withstood desiccation better than did their mannitol-grown equivalents. Rhizobia that were grown on whey and then inoculated onto commercial peat showed a survival rate of 100% after 23 weeks at -4 degrees C. Whey-grown cells in peat performed better at various temperatures during storage, even when they were exposed to desiccation, than did mannitol-grown cells in peat. Whey, therefore, offers interesting possibilities as a Rhizobium protectant for the inoculum industry.