| Literature DB >> 15052419 |
M J Estrella1, F L Pieckenstain, M Marina, L E Díaz, O A Ruiz.
Abstract
Cheese whey (CW)-based growth medium efficiently protects Rhizobium loti cells during freezing and desiccation and can maintain their growth in a manner similar to that of traditional mannitol-based medium (YEM). The cheese-whey-based medium (CW) improved viability when used to re-suspend cell pellets kept at -20 degrees C and -80 degrees C and resulted in the survival of over 90% of the cells. Moreover, bacterial pellets obtained from cells grown in CW withstand desiccation better than cells grown in YEM. Survival was over 60% after 30 days at 4 degrees C. No differences were observed in nodulation efficiency between YEM-grown and CW-grown cells. Fast protein liquid chromatography (FPLC) protocols are presented for total protein profile analyses of sweet and acid cheese whey.Entities:
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Year: 2004 PMID: 15052419 DOI: 10.1007/s10295-004-0124-y
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN: 1367-5435 Impact factor: 3.346