| Literature DB >> 1611672 |
Abstract
The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wild-type, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.Entities:
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Year: 1992 PMID： 1611672 DOI： 10.1007/bf00351735
Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886