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Literature DB >> 11454743

A genetic look at the active site of RNA polymerase III.

Abstract

rpc160-112, a mutant of the RNA polymerase III active site, is corrected in vivo by six second-site mutants obtained by random mutagenesis. These mutants introduce single-site amino acid replacements at the two large subunits of the enzyme. The mutated motifs are conserved in RNA polymerases I and II and, for some of them, in the bacterial enzyme, thus delineating key elements of the active site in eukaryotic RNA polymerases.

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Year: 2001 PMID： 11454743 PMCID： PMC1083950 DOI： 10.1093/embo-reports/kve136

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

28 in total

1. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution.

Authors: G Zhang; E A Campbell; L Minakhin; C Richter; K Severinov; S A Darst
Journal: Cell Date: 1999-09-17 Impact factor: 41.582

2. Functional characterization of ABC10alpha, an essential polypeptide shared by all three forms of eukaryotic DNA-dependent RNA polymerases.

Authors: L Rubbi; S Labarre-Mariotte; S Chédin; P Thuriaux
Journal: J Biol Chem Date: 1999-10-29 Impact factor: 5.157

3. A structural model of transcription elongation.

Authors: N Korzheva; A Mustaev; M Kozlov; A Malhotra; V Nikiforov; A Goldfarb; S A Darst
Journal: Science Date: 2000-07-28 Impact factor: 47.728

4. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system.

Authors: M Grenson; M Mousset; J M Wiame; J Bechet
Journal: Biochim Biophys Acta Date: 1966-10-31

A genetic look at the active site of RNA polymerase III.

1. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution.

2. Functional characterization of ABC10alpha, an essential polypeptide shared by all three forms of eukaryotic DNA-dependent RNA polymerases.

3. A structural model of transcription elongation.

4. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system.

5. Genetic interactions within TFIIIC, the promoter-binding factor of yeast RNA polymerase III.

6. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae.

7. Determination of lysine residues affinity labeled in the active site of yeast RNA polymerase II(B) by mutagenesis.

8. RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C.

9. Extranuclear gene expression in yeast: evidence for a plasmid-encoded RNA polymerase of unique structure.

10. Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase.

Review 1. Structural perspective on mutations affecting the function of multisubunit RNA polymerases.

2. Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases.

3. Kinetics of nucleotide entry into RNA polymerase active site provides mechanism for efficiency and fidelity.

4. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae.

Review 5. Non-canonical DNA transcription enzymes and the conservation of two-barrel RNA polymerases.

6. Maf1 is involved in coupling carbon metabolism to RNA polymerase III transcription.