Literature DB >> 1583656

Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.

N P Carter1, M A Ferguson-Smith, M T Perryman, H Telenius, A H Pelmear, M A Leversha, M T Glancy, S L Wood, K Cook, H M Dyson.   

Abstract

We describe a method, termed reverse chromosome painting, which allows the rapid analysis of the content and breakpoints of aberrant chromosomes. The method involves the sorting of small numbers of the aberrant chromosome from short term blood culture preparations or cell lines by using bivariate flow karyotype analysis. The sorted chromosomes are amplified and biotin labelled enzymatically using a degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), the product annealed to metaphase spreads from normal subjects, and hybridisation detected using fluorescence in situ hybridisation (FISH). We show the usefulness of this method for routine clinical cytogenetics by the analysis of cases involving an insertion, a deletion, a translocation, and two cases of a chromosome with additional material of unknown origin. The method has particular application for the rapid resolution of the origin of de novo unbalanced chromosome duplications.

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Year:  1992        PMID： 1583656      PMCID： PMC1015948          DOI： 10.1136/jmg.29.5.299

Source DB:  PubMed          Journal:  J Med Genet        ISSN： 0022-2593            Impact factor:   6.318


  11 in total

1.  Prenatal diagnosis of a double bisatellited marker with an unusual copy number ratio.

Authors:  J J Waters; M E Ferguson-Smith; N Carter; J L Alexander; S V Hodgson
Journal:  Prenat Diagn       Date:  1990-10       Impact factor: 3.050

2.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

3.  Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.

Authors:  A Jauch; C Daumer; P Lichter; J Murken; T Schroeder-Kurth; T Cremer
Journal:  Hum Genet       Date:  1990-07       Impact factor: 4.132

4.  Chromosome in situ suppression hybridisation in clinical cytogenetics.

Authors:  M A Hulten; C P Gould; A S Goldman; J J Waters
Journal:  J Med Genet       Date:  1991-09       Impact factor: 6.318

5.  Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots.

Authors:  N P Carter; M E Ferguson-Smith; N A Affara; H Briggs; M A Ferguson-Smith
Journal:  Cytometry       Date:  1990

6.  Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.

Authors:  P Lichter; T Cremer; J Borden; L Manuelidis; D C Ward
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

7.  Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.

Authors:  T Cremer; P Lichter; J Borden; D C Ward; L Manuelidis
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

8.  Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors:  D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

9.  Rapid detection of human chromosome 21 aberrations by in situ hybridization.

Authors:  P Lichter; T Cremer; C J Tang; P C Watkins; L Manuelidis; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

10.  Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes.

Authors:  H Telenius; A H Pelmear; A Tunnacliffe; N P Carter; A Behmel; M A Ferguson-Smith; M Nordenskjöld; R Pfragner; B A Ponder
Journal:  Genes Chromosomes Cancer       Date:  1992-04       Impact factor: 5.006
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  55 in total

1.  An easy and reliable procedure of microdissection technique for the analysis of chromosomal breakpoints and marker chromosomes.

Authors:  J Weimer; M Kiechle; G Senger; U Wiedemann; A Ovens-Raeder; S Schuierer; M Kautza; R Siebert; N Arnold
Journal:  Chromosome Res       Date:  1999       Impact factor: 5.239

2.  Comparative chromosome map of the laboratory mouse and Chinese hamster defined by reciprocal chromosome painting.

Authors:  F Yang; P C O'Brien; M A Ferguson-Smith
Journal:  Chromosome Res       Date:  2000       Impact factor: 5.239

3.  A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.

Authors:  Yeun-Jun Chung; Jos Jonkers; Hannah Kitson; Heike Fiegler; Sean Humphray; Carol Scott; Sarah Hunt; Yuejin Yu; Ichiko Nishijima; Arno Velds; Henne Holstege; Nigel Carter; Allan Bradley
Journal:  Genome Res       Date:  2004-01       Impact factor: 9.043

4.  Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays.

Authors:  H Fiegler; S M Gribble; D C Burford; P Carr; E Prigmore; K M Porter; S Clegg; J A Crolla; N R Dennis; P Jacobs; N P Carter
Journal:  J Med Genet       Date:  2003-09       Impact factor: 6.318

5.  Applications of combined DNA microarray and chromosome sorting technologies.

Authors:  S M Gribble; H Fiegler; D C Burford; E Prigmore; F Yang; P Carr; B L Ng; T Sun; E S Kamberov; V L Makarov; J P Langmore; N P Carter
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

6.  Introduction: chromosomes go with the flow.

Authors:  Jaroslav Dolezel; Nigel Carter; Malcolm Ferguson-Smith
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

Review 7.  Multicolor chromosome painting in diagnostic and research applications.

Authors:  Sabine Langer; Jürgen Kraus; Isabell Jentsch; Michael R Speicher
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

Review 8.  Reverse painting highlights the origin of chromosome aberrations.

Authors:  Elisabeth Blennow
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

9.  Highly comprehensive karyotype analysis by a combination of spectral karyotyping (SKY), microdissection, and reverse painting (SKY-MD).

Authors:  J Weimer; M R Koehler; U Wiedemann; P Attermeyer; A Jacobsen; D Karow; M Kiechl; W Jonat; N Arnold
Journal:  Chromosome Res       Date:  2001       Impact factor: 5.239

10.  Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype.

Authors:  Julio S Masabanda; Dave W Burt; Patricia C M O'Brien; Alain Vignal; Valerie Fillon; Philippa S Walsh; Helen Cox; Helen G Tempest; Jacqueline Smith; Felix Habermann; Michael Schmid; Yoichi Matsuda; Malcolm A Ferguson-Smith; Richard P M A Crooijmans; Martien A M Groenen; Darren K Griffin
Journal:  Genetics       Date:  2004-03       Impact factor: 4.562
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