Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A new activity for an old enzyme: Escherichia coli bacterial alkaline phosphatase is a phosphite-dependent hydrogenase.

Literature DB >> 15148399

A new activity for an old enzyme: Escherichia coli bacterial alkaline phosphatase is a phosphite-dependent hydrogenase.

Abstract

Genetic analysis indicates that Escherichia coli possesses two independent pathways for oxidation of phosphite (Pt) to phosphate. One pathway depends on the 14-gene phn operon, which encodes the enzyme C-P lyase. The other pathway depends on the phoA locus, which encodes bacterial alkaline phosphatase (BAP). Transposon mutagenesis studies strongly suggest that BAP is the only enzyme involved in the phoA-dependent pathway. This conclusion is supported by purification and biochemical characterization of the Pt-oxidizing enzyme, which was proven to be BAP by N terminus protein sequencing. Highly purified BAP catalyzed Pt oxidation with specific activities of 62-242 milliunits/mg and phosphate ester hydrolysis with specific activities of 41-61 units/mg. Surprisingly, BAP catalyzes the oxidation of Pt to phosphate and molecular H2. Thus, BAP is a unique Pt-dependent, H2-evolving hydrogenase. This reaction is unprecedented in both P and H biochemistry, and it is likely to involve direct transfer of hydride from the substrate to water-derived protons.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2004 PMID： 15148399 PMCID： PMC419532 DOI： 10.1073/pnas.0400664101

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

36 in total

1. Basic local alignment search tool.

Authors: S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal: J Mol Biol Date: 1990-10-05 Impact factor: 5.469

2. An improved assay for nanomole amounts of inorganic phosphate.

Authors: P A Lanzetta; L J Alvarez; P S Reinach; O A Candia
Journal: Anal Biochem Date: 1979-11-15 Impact factor: 3.365

3. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

Authors: W W Metcalf; P M Steed; B L Wanner
Journal: J Bacteriol Date: 1990-06 Impact factor: 3.490

4. Bidirectional chain-termination nucleotide sequencing: transposon Tn5seq1 as a mobile source of primer sites.

Authors: D K Nag; H V Huang; D E Berg
Journal: Gene Date: 1988-04-15 Impact factor: 3.688

5. Anaerobic utilization of phosphite and hypophosphite by Bacillus sp.

Authors: T L Foster; L Winans; S J Helms
Journal: Appl Environ Microbiol Date: 1978-05 Impact factor: 4.792

6. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12.

Authors: B L Wanner
Journal: J Mol Biol Date: 1986-09-05 Impact factor: 5.469

7. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli.

Authors: B L Wanner; J A Boline
Journal: J Bacteriol Date: 1990-03 Impact factor: 3.490

8. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi.

Authors: W W Metcalf; B L Wanner
Journal: J Bacteriol Date: 1991-01 Impact factor: 3.490

9. Identification of a protein required for disulfide bond formation in vivo.

Authors: J C Bardwell; K McGovern; J Beckwith
Journal: Cell Date: 1991-11-01 Impact factor: 41.582

10. Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure.

Authors: F M Hulett; E E Kim; C Bookstein; N V Kapp; C W Edwards; H W Wyckoff
Journal: J Biol Chem Date: 1991-01-15 Impact factor: 5.157

43 in total

1. The Streptomyces-produced antibiotic fosfomycin is a promiscuous substrate for archaeal isopentenyl phosphate kinase.

Authors: Mark F Mabanglo; Adrian W R Serohijos; C Dale Poulter
Journal: Biochemistry Date: 2012-01-11 Impact factor: 3.162

Review 2. Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases.

Authors: Mikael Elias; Dan S Tawfik
Journal: J Biol Chem Date: 2011-11-08 Impact factor: 5.157

3. The subtle benefits of being promiscuous: adaptive evolution potentiated by enzyme promiscuity.

Authors: Mark A Depristo
Journal: HFSP J Date: 2007-07-10

4. Metagenomics-guided analysis of microbial chemolithoautotrophic phosphite oxidation yields evidence of a seventh natural CO₂ fixation pathway.

Authors: Israel A Figueroa; Tyler P Barnum; Pranav Y Somasekhar; Charlotte I Carlström; Anna L Engelbrektson; John D Coates
Journal: Proc Natl Acad Sci U S A Date: 2017-11-28 Impact factor: 11.205

5. An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily.

Authors: Bert van Loo; Stefanie Jonas; Ann C Babtie; Alhosna Benjdia; Olivier Berteau; Marko Hyvönen; Florian Hollfelder
Journal: Proc Natl Acad Sci U S A Date: 2010-01-27 Impact factor: 11.205

6. Identification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans.

Authors: Diliana Dancheva Simeonova; Marlena Marie Wilson; William W Metcalf; Bernhard Schink
Journal: J Bacteriol Date: 2010-07-09 Impact factor: 3.490

A new activity for an old enzyme: Escherichia coli bacterial alkaline phosphatase is a phosphite-dependent hydrogenase.

1. Basic local alignment search tool.

2. An improved assay for nanomole amounts of inorganic phosphate.

3. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

4. Bidirectional chain-termination nucleotide sequencing: transposon Tn5seq1 as a mobile source of primer sites.

5. Anaerobic utilization of phosphite and hypophosphite by Bacillus sp.

6. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12.

7. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli.

8. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi.

9. Identification of a protein required for disulfide bond formation in vivo.

10. Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure.

1. The Streptomyces-produced antibiotic fosfomycin is a promiscuous substrate for archaeal isopentenyl phosphate kinase.

Review 2. Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases.

3. The subtle benefits of being promiscuous: adaptive evolution potentiated by enzyme promiscuity.

4. Metagenomics-guided analysis of microbial chemolithoautotrophic phosphite oxidation yields evidence of a seventh natural CO₂ fixation pathway.

5. An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily.

6. Identification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans.

7. Redox chemistry in the phosphorus biogeochemical cycle.

8. AcpA is a Francisella acid phosphatase that affects intramacrophage survival and virulence.

Review 9. Catalytic promiscuity and heme-dependent redox regulation of H₂S synthesis.

10. Uptake of glycerol-2-phosphate via the ugp-encoded transporter in Escherichia coli K-12.

Review 2. Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases.

Review 9. Catalytic promiscuity and heme-dependent redox regulation of H2S synthesis.

Review 9. Catalytic promiscuity and heme-dependent redox regulation of H₂S synthesis.