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Literature DB >> 14551782

Validation of the multiplex kit genRESMPX-2 for forensic casework analysis.

A Junge¹, T Lederer, G Braunschweiger, B Madea.

Abstract

Validation studies were carried out using the commercially available PCR multiplex system genRESMPX-2. In addition to amelogenin, this system comprises the complete set of eight STR systems which are components of the German DNA database established in 1998 by the Federal Criminal Office of Germany (BKA). The minimum amount of template DNA which gave a complete DNA pattern ranged between 100 pg and 200 pg. Mixed samples could clearly be assigned from ratios between 1:5 (ACTBP2) and 1:20 (VWA, FGA). Experimental investigations with different forensic materials, environmental studies, reproducibility and precision data as well as practical casework analysis revealed that the genRESMPX-2 kit can be regarded as a sensitive, reliable and robust multiplex system even in the case of samples containing limited amounts or degraded DNA.

Entities: Gene

Mesh：

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Year: 2003 PMID： 14551782 DOI： 10.1007/s00414-003-0385-4

Source DB: PubMed Journal: Int J Legal Med ISSN： 0937-9827 Impact factor: 2.686

21 in total

1. A new pentaplex PCR system for forensic casework analysis.

Authors: T Lederer; S Seidl; B Graham; P Betz
Journal: Int J Legal Med Date: 2000 Impact factor: 2.686

2. Validation of the AMPFlSTR SGM plus system for use in forensic casework.

Authors: E A Cotton; R F Allsop; J L Guest; R R Frazier; P Koumi; I P Callow; A Seager; R L Sparkes
Journal: Forensic Sci Int Date: 2000-08-14 Impact factor: 2.395

3. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples.

Authors: T R Moretti; A L Baumstark; D A Defenbaugh; K M Keys; J B Smerick; B Budowle
Journal: J Forensic Sci Date: 2001-05 Impact factor: 1.832

Review 4. The GEDNAP (German DNA profiling group) blind trial concept.

Authors: Steven Rand; Marianne Schürenkamp; Bernd Brinkmann
Journal: Int J Legal Med Date: 2002-03-28 Impact factor: 2.686

5. Multiplex sets for the amplification of polymorphic short tandem repeat loci--silver stain and fluorescence detection.

Authors: A M Lins; C J Sprecher; C Puers; J W Schumm
Journal: Biotechniques Date: 1996-05 Impact factor: 1.993

6. A simple salting out procedure for extracting DNA from human nucleated cells.

Authors: S A Miller; D D Dykes; H F Polesky
Journal: Nucleic Acids Res Date: 1988-02-11 Impact factor: 16.971

7. Automated DNA profiling employing multiplex amplification of short tandem repeat loci.

Authors: C P Kimpton; P Gill; A Walton; A Urquhart; E S Millican; M Adams
Journal: PCR Methods Appl Date: 1993-08

8. Highly discriminating heptaplex short tandem repeat PCR system for forensic identification.

Authors: A Urquhart; N J Oldroyd; C P Kimpton; P Gill
Journal: Biotechniques Date: 1995-01 Impact factor: 1.993

9. Sequence variation in humans and other primates at six short tandem repeat loci used in forensic identity testing.

Authors: K Lazaruk; J Wallin; C Holt; T Nguyen; P S Walsh
Journal: Forensic Sci Int Date: 2001-06-01 Impact factor: 2.395

10. Validation and population studies of the loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQ alpha using a multiplex amplification and typing procedure.

Authors: B Budowle; J A Lindsey; J A DeCou; B W Koons; A M Giusti; C T Comey
Journal: J Forensic Sci Date: 1995-01 Impact factor: 1.832

12 in total

1. The GEDNAP blind trial concept part II. Trends and developments.

Authors: S Rand; M Schürenkamp; C Hohoff; B Brinkmann
Journal: Int J Legal Med Date: 2004-01-31 Impact factor: 2.686

2. Development of a 13-locus PCR multiplex system for paternity testing.

Authors: J Schlenk; S Seidl; G Braunschweiger; P Betz; T Lederer
Journal: Int J Legal Med Date: 2004-01-14 Impact factor: 2.686

3. Development of the AmpFISTR SEfiler PCR amplification kit: a new multiplex containing the highly discriminating ACTBP2 (SE33) locus.

Authors: Sulekha Rao Coticone; Nicola Oldroyd; Heidi Philips; Paul Foxall
Journal: Int J Legal Med Date: 2004-05-15 Impact factor: 2.686

Validation of the multiplex kit genRESMPX-2 for forensic casework analysis.

1. A new pentaplex PCR system for forensic casework analysis.

2. Validation of the AMPFlSTR SGM plus system for use in forensic casework.

3. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples.

Review 4. The GEDNAP (German DNA profiling group) blind trial concept.

5. Multiplex sets for the amplification of polymorphic short tandem repeat loci--silver stain and fluorescence detection.

6. A simple salting out procedure for extracting DNA from human nucleated cells.

7. Automated DNA profiling employing multiplex amplification of short tandem repeat loci.

8. Highly discriminating heptaplex short tandem repeat PCR system for forensic identification.

9. Sequence variation in humans and other primates at six short tandem repeat loci used in forensic identity testing.

10. Validation and population studies of the loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQ alpha using a multiplex amplification and typing procedure.

1. The GEDNAP blind trial concept part II. Trends and developments.

2. Development of a 13-locus PCR multiplex system for paternity testing.

3. Development of the AmpFISTR SEfiler PCR amplification kit: a new multiplex containing the highly discriminating ACTBP2 (SE33) locus.

4. Allelic drop-out in the STR system ACTBP2 (SE33) as a result of mutations in the primer binding region.

5. A validation study of the Qiagen Investigator DIPplex® kit; an INDEL-based assay for human identification.

6. Unusual variant alleles in commonly used short tandem repeat loci.

7. Short amplicon STR multiplex for stain typing.

8. Wonder or fake - investigations in the case of the stigmatisation of Therese Neumann von Konnersreuth.

9. Mutations or exclusion: an unusual case in paternity testing.

10. Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit.