Literature DB >> 1408787

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

P Schmieder1, J H Ippel, H van den Elst, G A van der Marel, J H van Boom, C Altona, H Kessler.   

Abstract

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

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Year:  1992        PMID： 1408787      PMCID： PMC334227          DOI： 10.1093/nar/20.18.4747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Determination of the DNA sugar pucker using 13C NMR spectroscopy.

Authors:  R A Santos; P Tang; G S Harbison
Journal:  Biochemistry       Date:  1989-11-28       Impact factor: 3.162

2.  Heteronuclear nuclear magnetic resonance experiments for studies of protein conformation.

Authors:  G Wagner
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

3.  Concerted two-dimensional NMR approaches to hydrogen-1, carbon-13, and nitrogen-15 resonance assignments in proteins.

Authors:  B J Stockman; M D Reily; W M Westler; E L Ulrich; J L Markley
Journal:  Biochemistry       Date:  1989-01-10       Impact factor: 3.162

Review 4.  Biosynthetic incorporation of 15N and 13C for assignment and interpretation of nuclear magnetic resonance spectra of proteins.

Authors:  L P McIntosh; F W Dahlquist
Journal:  Q Rev Biophys       Date:  1990-02       Impact factor: 5.318

5.  Determination of heteronuclear long-range couplings to heteronuclei in natural abundance by two- and three-dimensional NMR spectroscopy.

Authors:  P Schmieder; M Kurz; H Kessler
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

6.  13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation.

Authors:  S R LaPlante; J Ashcroft; D Cowburn; G C Levy; P N Borer
Journal:  J Biomol Struct Dyn       Date:  1988-04

7.  Carbon-13 NMR in conformational analysis of nucleic acid fragments. Heteronuclear chemical shift correlation spectroscopy of RNA constituents.

Authors:  P P Lankhorst; C Erkelens; C A Haasnoot; C Altona
Journal:  Nucleic Acids Res       Date:  1983-10-25       Impact factor: 16.971

8.  Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.

Authors:  W Leupin; G Wagner; W A Denny; K Wüthrich
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

9.  13C NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features.

Authors:  S R LaPlante; E A Boudreau; N Zanatta; G C Levy; P N Borer; J Ashcroft; D Cowburn
Journal:  Biochemistry       Date:  1988-10-04       Impact factor: 3.162

10.  A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors:  M Ikura; L E Kay; A Bax
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
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  10 in total

1.  Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.

Authors:  A T Phan
Journal:  J Biomol NMR       Date:  2000-02       Impact factor: 2.835

2.  Stereospecific assignment of H5' and H5″ in a (5'R)-/(5'S)-deuterium- labeled DNA decamer for(3) J (HH) determination and unambiguous NOE assignments.

Authors:  C Kojima; E Kawashima; K Toyama; K Ohshima; Y Ishido; M Kainosho; Y Kyogoku
Journal:  J Biomol NMR       Date:  1998-01       Impact factor: 2.835

3.  Enhanced loop DNA folding induced by thymine-CH3 group contact and perpendicular guanine-thymine interaction.

Authors:  S H Cho; K H Chin; C W Chen
Journal:  J Biomol NMR       Date:  2001-01       Impact factor: 2.835

4.  Tuning of conformational preorganization in model 2',5'- and 3',5'-linked oligonucleotides by 3'- and 2'-O-methoxyethyl modification.

Authors:  Matjaz Polak; Muthiah Manoharan; Gopal B Inamati; Janez Plavec
Journal:  Nucleic Acids Res       Date:  2003-04-15       Impact factor: 16.971

5.  Natural abundance heteronuclear NMR studies of the T3 mini-loop hairpin in the terminal repeat of the adenoassociated virus 2.

Authors:  S H Chou; Y Y Tseng; B Y Chu
Journal:  J Biomol NMR       Date:  2000-05       Impact factor: 2.835

6.  Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.

Authors:  G Varani; F Aboul-ela; F Allain; C C Gubser
Journal:  J Biomol NMR       Date:  1995-04       Impact factor: 2.835

7.  Conformation of the circular dumbbell d<pCGC-TT-GCG-TT>: structure determination and molecular dynamics.

Authors:  J H Ippel; V Lanzotti; A Galeone; L Mayol; J E van den Boogaart; J A Pikkemaat; C Altona
Journal:  J Biomol NMR       Date:  1995-12       Impact factor: 2.835

8.  Structural characterization of d(CAACCCGTTG) and d(CAACGGGTTG) mini-hairpin loops by heteronuclear NMR: the effects of purines versus pyrimidines in DNA hairpins.

Authors:  D Z Avizonis; D R Kearns
Journal:  Nucleic Acids Res       Date:  1995-04-11       Impact factor: 16.971

9.  Preparation and heteronuclear 2D NMR spectroscopy of a DNA dodecamer containing a thymidine residue with a uniformly 13C-labeled deoxyribose ring.

Authors:  A Ono; S Tate; Y Ishido; M Kainosho
Journal:  J Biomol NMR       Date:  1994-07       Impact factor: 2.835

10.  Determination of the glycosidic torsion angles in uniformly 13C-labeled nucleic acids from vicinal coupling constants 3J(C2)/4-H1' and 3J(C6)/8-H1'.

Authors:  Lukás Trantírek; Richard Stefl; James E Masse; Juli Feigon; Vladimír Sklenár
Journal:  J Biomol NMR       Date:  2002-05       Impact factor: 2.835
  10 in total

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