Literature DB >> 20859785

Stereospecific assignment of H5' and H5″ in a (5'R)-/(5'S)-deuterium- labeled DNA decamer for(3) J (HH) determination and unambiguous NOE assignments.

C Kojima1, E Kawashima, K Toyama, K Ohshima, Y Ishido, M Kainosho, Y Kyogoku.   

Abstract

The stereoselective deuterium labeling at the 5' methylene protons of the ribose ring recently developed by Kawashima et al. [1995, Tetrahedron Lett., 36, 6699-6700] enabled the assignment of pro-R and pro-S protons at the 5' position. The deuterium-labeled nucleotides, [(5'S)-(2)H]- and [(5'R)-(2)H]-diastereomers, in an approximate ratio of 2:1, were incorporated in the decamer 5'-d(GCATTAATGC)-3'. Thus, both pro-R and pro-S methylene proton signals without geminal coupling appeared in the NOESY and DQF-COSY spectra. Complete stereospecific assignments and simplified spin systems enabled the determination of 15 (3)J coupling constants between H4' and H5'/H5", and the unambiguous assignment of 135 NOESY cross peaks originating from H4'/H5'/H5" resonances.

Entities:  

Year:  1998        PMID： 20859785     DOI： 10.1023/A:1008286925516

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  11 in total

1.  Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

Authors:  P Schmieder; J H Ippel; H van den Elst; G A van der Marel; J H van Boom; C Altona; H Kessler
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

2.  The octamer motif in immunoglobulin genes: extraction of structural constraints from two-dimensional NMR studies.

Authors:  K Weisz; R H Shafer; W Egan; T L James
Journal:  Biochemistry       Date:  1992-08-25       Impact factor: 3.162

Review 3.  RNA structure and NMR spectroscopy.

Authors:  G Varani; I Tinoco
Journal:  Q Rev Biophys       Date:  1991-11       Impact factor: 5.318

4.  Complete assignment of the deoxyribose 5'/5" proton resonances of the EcoRI DNA sequence using isotropic mixing.

Authors:  S J Glaser; M L Remerowski; G P Drobny
Journal:  Biochemistry       Date:  1989-02-21       Impact factor: 3.162

Review 5.  Nucleic acids and nuclear magnetic resonance.

Authors:  F J Van de Ven; C W Hilbers
Journal:  Eur J Biochem       Date:  1988-12-01

6.  1H nuclear magnetic resonance assignments for d-(GCATTAATGC)2 using experimental refinements of established procedures.

Authors:  W J Chazin; K Wüthrich; S Hyberts; M Rance; W A Denny; W Leupin
Journal:  J Mol Biol       Date:  1986-08-05       Impact factor: 5.469

7.  Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra.

Authors:  M Remin; D Shugar
Journal:  Biochem Biophys Res Commun       Date:  1972-08-07       Impact factor: 3.575

8.  Induced structural changes in protein-DNA complexes.

Authors:  Y Kyogoku; C Kojima; S J Lee; H Tochio; N Suzuki; H Matsuo; M Shirakawa
Journal:  Methods Enzymol       Date:  1995       Impact factor: 1.600

9.  The three-dimensional structure of a DNA hairpin in solution two-dimensional NMR studies and structural analysis of d(ATCCTATTTATAGGAT).

Authors:  M J Blommers; F J van de Ven; G A van der Marel; J H van Boom; C W Hilbers
Journal:  Eur J Biochem       Date:  1991-10-01

10.  Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2.

Authors:  D Neuhaus; G Wagner; M Vasák; J H Kägi; K Wüthrich
Journal:  Eur J Biochem       Date:  1985-09-02
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  1 in total

1.  Sugar conformation of a stereospecific 2'-R or 2'-S deuterium-labeled DNA decamer studied with proton-proton J coupling constants.

Authors:  C Kojima; E Kawashima; T Sekine; Y Ishido; A Ono; M Kainosho; Y Kyogoku
Journal:  J Biomol NMR       Date:  2001-01       Impact factor: 2.835
  1 in total

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