Literature DB >> 1374836

Detection of a CpA methylase in an insect system: characterization and substrate specificity.

C Devajyothi1, V Brahmachari.   

Abstract

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependence similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

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Year:  1992        PMID： 1374836     DOI： 10.1007/bf02454187

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  31 in total

1.  DNA modification mechanisms and gene activity during development.

Authors:  R Holliday; J E Pugh
Journal:  Science       Date:  1975-01-24       Impact factor: 47.728

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Eukaryotic DNA methylase--properties and action on native DNA and chromatin.

Authors:  R L Adams; T Davis; J Fulton; D Kirk; M Qureshi; R H Burdon
Journal:  Curr Top Microbiol Immunol       Date:  1984       Impact factor: 4.291

4.  Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects.

Authors:  P M Rae; R E Steele
Journal:  Nucleic Acids Res       Date:  1979-07-11       Impact factor: 16.971

5.  DNA methylase from HeLa cell nuclei.

Authors:  P H Roy; A Weissbach
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

6.  DNA-methylase from regenerating rat liver: purification and characterisation.

Authors:  D Simon; F Grunert; U von Acken; H P Döring; H Kröger
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

7.  Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II.

Authors:  J Singer; J Roberts-Ems; A D Riggs
Journal:  Science       Date:  1979-03-09       Impact factor: 47.728

8.  Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA.

Authors:  C W Achwal; C A Iyer; H S Chandra
Journal:  FEBS Lett       Date:  1983-07-25       Impact factor: 4.124

9.  Isolation and characterization of DNA cytosine 5-methyltransferase from human placenta.

Authors:  G P Pfeifer; S Grünwald; T L Boehm; D Drahovsky
Journal:  Biochim Biophys Acta       Date:  1983-08-02

10.  Mouse ascites DNA methylase: characterisation of size, proteolytic breakdown and nucleotide recognition.

Authors:  R L Adams; K Gardiner; A Rinaldi; M Bryans; M McGarvey; R H Burdon
Journal:  Biochim Biophys Acta       Date:  1986-10-16
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  4 in total

1.  The relationship between DNA methylation and chromosome imprinting in the coccid Planococcus citri.

Authors:  S Bongiorni; O Cintio; G Prantera
Journal:  Genetics       Date:  1999-04       Impact factor: 4.562

Review 2.  In praise of mealybugs.

Authors:  Vani Brahmachari; Surbhi Kohli; Parul Gulati
Journal:  J Genet       Date:  2018-06       Impact factor: 1.166

3.  DNA of Drosophila melanogaster contains 5-methylcytosine.

Authors:  H Gowher; O Leismann; A Jeltsch
Journal:  EMBO J       Date:  2000-12-15       Impact factor: 11.598

4.  Evolution of DNA Methylation Across Ecdysozoa.

Authors:  Jan Engelhardt; Oliver Scheer; Peter F Stadler; Sonja J Prohaska
Journal:  J Mol Evol       Date:  2022-01-28       Impact factor: 2.395
  4 in total

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