Literature DB >> 11118227

DNA of Drosophila melanogaster contains 5-methylcytosine.

H Gowher1, O Leismann, A Jeltsch.   

Abstract

It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [gamma-(32)P]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D. melanogaster at a level of approximately 1 in 1000-2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 11118227      PMCID： PMC305887          DOI： 10.1093/emboj/19.24.6918

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  47 in total

1.  Vestiges of a DNA methylation system in Drosophila melanogaster?

Authors:  S Tweedie; H H Ng; A L Barlow; B M Turner; B Hendrich; A Bird
Journal:  Nat Genet       Date:  1999-12       Impact factor: 38.330

Review 2.  Genomic imprinting in mammals: an interplay between chromatin and DNA methylation?

Authors:  R Feil; S Khosla
Journal:  Trends Genet       Date:  1999-11       Impact factor: 11.639

Review 3.  Relationships between chromatin organization and DNA methylation in determining gene expression.

Authors:  P L Jones; A P Wolffe
Journal:  Semin Cancer Biol       Date:  1999-10       Impact factor: 15.707

4.  Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene.

Authors:  G L Xu; T H Bestor; D Bourc'his; C L Hsieh; N Tommerup; M Bugge; M Hulten; X Qu; J J Russo; E Viegas-Péquignot
Journal:  Nature       Date:  1999-11-11       Impact factor: 49.962

Review 5.  CpG island methylator phenotypes in aging and cancer.

Authors:  M Toyota; J P Issa
Journal:  Semin Cancer Biol       Date:  1999-10       Impact factor: 15.707

6.  Mammalian (cytosine-5) methyltransferases cause genomic DNA methylation and lethality in Drosophila.

Authors:  F Lyko; B H Ramsahoye; H Kashevsky; M Tudor; M A Mastrangelo; T L Orr-Weaver; R Jaenisch
Journal:  Nat Genet       Date:  1999-11       Impact factor: 38.330

7.  On the substrate specificity of DNA methyltransferases. adenine-N6 DNA methyltransferases also modify cytosine residues at position N4.

Authors:  A Jeltsch; F Christ; M Fatemi; M Roth
Journal:  J Biol Chem       Date:  1999-07-09       Impact factor: 5.157

8.  The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome.

Authors:  R S Hansen; C Wijmenga; P Luo; A M Stanek; T K Canfield; C M Weemaes; S M Gartler
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

9.  Cloning and characterization of the multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster.

Authors:  M Johansson; A R van Rompay; B Degrève; J Balzarini; A Karlsson
Journal:  J Biol Chem       Date:  1999-08-20       Impact factor: 5.157

10.  DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.

Authors:  M Okano; D W Bell; D A Haber; E Li
Journal:  Cell       Date:  1999-10-29       Impact factor: 41.582
View more
  61 in total

Review 1.  The rest is silence.

Authors:  E Bernstein; A M Denli; G J Hannon
Journal:  RNA       Date:  2001-11       Impact factor: 4.942

2.  De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae.

Authors:  Stefan Juranek; Hans-Joachim Wieden; Hans J Lipps
Journal:  Nucleic Acids Res       Date:  2003-03-01       Impact factor: 16.971

3.  The inactive X chromosome in the human female is enriched in 5-methylcytosine to an unusual degree and appears to contain more of this modified nucleotide than the remainder of the genome.

Authors:  Deepti D Deobagkar; H Sharat Chandra
Journal:  J Genet       Date:  2003 Apr-Aug       Impact factor: 1.166

Review 4.  Gene silencing-based disease resistance.

Authors:  Michael Wassenegger
Journal:  Transgenic Res       Date:  2002-12       Impact factor: 2.788

5.  Epigenetics comes of age in the twentyfirst century.

Authors:  Robin Holliday
Journal:  J Genet       Date:  2002-04       Impact factor: 1.166

Review 6.  Eukaryotic methyl-CpG-binding domain proteins and chromatin modification.

Authors:  Ming-Shiu Hung; C-K James Shen
Journal:  Eukaryot Cell       Date:  2003-10

7.  Sex-specific methylation in Drosophila: an investigation of the Sophophora subgenus.

Authors:  Marícia Fantinel D'Avila; Rosane Nunes Garcia; Yanina Panzera; Vera Lúcia da Silva Valente
Journal:  Genetica       Date:  2010-07-17       Impact factor: 1.082

8.  DNA hypermethylation in Drosophila melanogaster causes irregular chromosome condensation and dysregulation of epigenetic histone modifications.

Authors:  Frank Weissmann; Inhua Muyrers-Chen; Tanja Musch; Dirk Stach; Manfred Wiessler; Renato Paro; Frank Lyko
Journal:  Mol Cell Biol       Date:  2003-04       Impact factor: 4.272

9.  Evolution of gene sequence in response to chromosomal location.

Authors:  Carlos Díaz-Castillo; Kent G Golic
Journal:  Genetics       Date:  2007-09       Impact factor: 4.562

10.  In silico prediction of two classes of honeybee genes with CpG deficiency or CpG enrichment and sorting according to gene ontology classes.

Authors:  Yong Wang; Frederick C C Leung
Journal:  J Mol Evol       Date:  2009-05-23       Impact factor: 2.395
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.